Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Food Chem. 2017 Jun 1;224:131-138. doi: 10.1016/j.foodchem.2016.12.024. Epub 2016 Dec 15.
Fabric phase sorptive extraction (FPSE), a novel sorbent-based microextraction method, was evaluated as a simple and rapid strategy for the extraction of four penicillin antibiotic residues (benzylpenicillin, cloxacillin, dicloxacillin and oxacillin) from cows' milk, without prior protein precipitation. Time-consuming solvent evaporation and reconstitution steps were eliminated successfully from the sample preparation workflow. FPSE utilizes a flexible fabric substrate, chemically coated with sol-gel derived, highly efficient, organic-inorganic hybrid sorbent as the extraction medium. Herein short-chain poly(ethylene glycol) provided optimum extraction sensitivity for the selected penicillins, which were analysed using an RP-HPLC method, validated according to the European Decision 657/2002/EC. The limit of quantitation was 10μg/kg for benzylpenicillin, 20μg/kg for cloxacillin, 25μg/kg dicloxacillin and 30μg/kg oxacillin. These are a similar order of magnitude with those reported in the literature and (with the exception of benzylpenicillin) are less than the maximum residue limits (MRL) set by European legislation.
纤维增强相固相萃取（FPSE）是一种新型的基于吸附剂的微萃取方法，被评估为一种从牛奶中提取四种青霉素抗生素残留（苄青霉素、氯唑西林、双氯西林和苯唑西林）的简单快速策略，无需预先进行蛋白质沉淀。成功地从样品制备工作流程中省去了耗时的溶剂蒸发和再构成步骤。FPSE 利用柔性纤维基底，化学涂覆溶胶-凝胶衍生的高效有机-无机杂化吸附剂作为萃取介质。在此，短链聚乙二醇（PEG）为所选青霉素提供了最佳的萃取灵敏度，采用反相高效液相色谱法（RP-HPLC）进行分析，并根据欧洲 657/2002/EC 号决定进行了验证。苄青霉素的定量限为 10μg/kg，氯唑西林为 20μg/kg，双氯西林为 25μg/kg，苯唑西林为 30μg/kg。这些与文献报道的数量级相似，并且（除了苄青霉素）均低于欧洲法规设定的最大残留限量（MRL）。
