Department of Conservative Dentistry, College of Dentistry, Baghdad University, Iraq; Department of Conservative Dentistry, King's College London Dental Institute, London, UK.
Department of Conservative Dentistry, King's College London Dental Institute, London, UK.
J Mech Behav Biomed Mater. 2017 May;69:355-361. doi: 10.1016/j.jmbbm.2017.01.022. Epub 2017 Jan 15.
To evaluate the bioactivity and the cytocompatibility of experimental Bioglass-reinforced polyethylene-based root-canal filling materials. The thermal properties of the experimental materials were also evaluated using differential scanning calorimetry, while their radiopacity was assessed using a grey-scale value (GSV) aluminium step wedge and a phosphor plate digital system. Bioglass 45S5 (BAG), polyethylene and Strontium oxide (SrO) were used to create tailored composite fibres. The filler distribution within the composites was assessed using SEM, while their bioactivity was evaluated through infrared spectroscopy (FTIR) after storage in simulated body fluid (SBF). The radiopacity of the composite fibres and their thermal properties were determined using differential scanning calorimetry (DSC). The cytocompatibility of the experimental composites used in this study was assessed using human osteoblasts and statistically analysed using the Pairwise t-test (p<0.05). Bioglass and SrO fillers were well distributed within the resin matrix and increased both the thermal properties and the radiopacity of the polyethylene matrix. The FTIR showed a clear formation of calcium-phosphates, while, MTT and AlamrBlue tests demonstrated no deleterious effects on the metabolic activity of the osteoblast-like cells. BAG-reinforced polyethylene composites may be suitable as obturation materials for endodontic treatment. Since their low melting temperature, such innovative composites may be easily removed in case of root canal retreatment. Moreover, their biocompatibility and bioactivity may benefit proliferation of human osteoblast cells at the periapical area of the root.
为了评估实验性 Bioglass 增强型聚乙烯基根管充填材料的生物活性和细胞相容性。还使用差示扫描量热法评估了实验材料的热性能，而其射线照相密度则使用灰度值（GSV）铝阶跃楔形物和磷光板数字系统进行评估。使用 45S5 生物玻璃（BAG）、聚乙烯和氧化锶（SrO）来制造定制的复合纤维。使用扫描电子显微镜（SEM）评估复合材料中的填充物分布，通过在模拟体液（SBF）中储存后使用红外光谱（FTIR）评估其生物活性。使用差示扫描量热法（DSC）确定复合纤维的射线照相密度和热性能。使用人成骨细胞评估本研究中使用的实验性复合材料的细胞相容性，并使用成对 t 检验（p<0.05）进行统计学分析。Bioglass 和 SrO 填料均匀分布在树脂基质中，提高了聚乙烯基质的热性能和射线照相密度。FTIR 显示出明显的钙磷形成，而 MTT 和 AlamrBlue 测试表明对成骨样细胞的代谢活性没有有害影响。BAG 增强型聚乙烯复合材料可用作牙髓治疗的封闭材料。由于其低熔点，这种创新的复合材料在根管再治疗的情况下很容易去除。此外，其生物相容性和生物活性可能有益于根尖区人成骨细胞的增殖。
