Pan J-X
Department of Cardiology, University-Town Hospital of Chongqing Medical University, Chongqing, China.
Eur Rev Med Pharmacol Sci. 2017 Jan;21(2):322-328.
Atherosclerosis is one of the leading causes of mortality in the world, which is a multistep cardiovascular disease promoted by several of risk factors. However, the detailed mechanism of atherosclerosis remained unclear. LncRNAs have been proved to play an important role in various of biological and pathological processes, but the role of lncRNA in atherosclerosis largely remained unidentified.
Blood sample were collected from 42 patients with atherosclerosis and 37 healthy volunteers. The expression of lncRNA H19 was detected by the qRT-PCR assay. Proliferation and apoptosis of HUVEC were also detected after lncRNA H19 was overexpressed. The expression of p38 and p65 were also measured by Western blot.
Compared with the normal healthy people, the expression of H19 was higher in patients with atherosclerosis. After lncRNA H19 was overexpressed in HUVEC, the proliferation ability was increased while apoptosis was suppressed. What's more, p38 and p65 were increased after lncRNA H19 was overexpressed.
LncRNA H19 was highly expressed in atherosclerosis, which could be used as a potential target for treating atherosclerosis.
动脉粥样硬化是全球主要死因之一，是一种由多种危险因素促成的多步骤心血管疾病。然而，动脉粥样硬化的详细机制仍不清楚。长链非编码RNA（lncRNAs）已被证明在各种生物和病理过程中发挥重要作用，但lncRNA在动脉粥样硬化中的作用在很大程度上仍未明确。
从42例动脉粥样硬化患者和37名健康志愿者中采集血样。通过qRT-PCR检测lncRNA H19的表达。在lncRNA H19过表达后，还检测了人脐静脉内皮细胞（HUVEC）的增殖和凋亡情况。通过蛋白质印迹法检测p38和p65的表达。
与正常健康人相比，动脉粥样硬化患者中H19的表达更高。在HUVEC中lncRNA H19过表达后，增殖能力增强而凋亡受到抑制。此外，lncRNA H19过表达后p38和p65增加。
LncRNA H19在动脉粥样硬化中高表达，可作为治疗动脉粥样硬化的潜在靶点。
