一种允许性细菌C-异戊二烯基转移酶的结构与特异性
Structure and specificity of a permissive bacterial C-prenyltransferase.
作者信息
Elshahawi Sherif I, Cao Hongnan, Shaaban Khaled A, Ponomareva Larissa V, Subramanian Thangaiah, Farman Mark L, Spielmann H Peter, Phillips George N, Thorson Jon S, Singh Shanteri
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.
Center for Pharmaceutical Research and Innovation (CPRI), University of Kentucky, Lexington, Kentucky, USA.
出版信息
Nat Chem Biol. 2017 Apr;13(4):366-368. doi: 10.1038/nchembio.2285. Epub 2017 Feb 6.
This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug.
本研究着重介绍了来自链霉菌属RM-5-8的L-色氨酸C6 C-异戊烯基转移酶（C-PT）PriB的生化和结构特征。研究发现，PriB对包括达托霉素在内的多种异戊烯基供体和受体具有独特的耐受性。另外两种异戊烯基转移酶也产生了新型的异戊烯基化达托霉素，其抗菌活性比母体药物有所提高。
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