脉冲光激发后氧合血红蛋白和碳氧血红蛋白的光谱研究。
Spectroscopic studies of oxy- and carbonmonoxyhemoglobin after pulsed optical excitation.
作者信息
Greene B I, Hochstrasser R M, Weisman R B, Eaton W A
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5255-9. doi: 10.1073/pnas.75.11.5255.
Abstract
The photolysis of HbO2 and HbCO has been investigated with picosecond laser techniques. Transient absorption spectra were measured in the Soret and visible regions after excitation with 353- or 530-nm pulses. The photoproducts appeared within 8 psec and exhibited considerably broadened deoxyhemoglobin-like spectra, which persisted to 680 psec. The altered spectra are attributed to the production of deoxyheme conformational and spin states that might result from the intense excitation.
摘要
已使用皮秒激光技术研究了 HbO₂ 和 HbCO 的光解。在用 353 纳米或 530 纳米脉冲激发后，在索雷特带和可见光区域测量了瞬态吸收光谱。光产物在 8 皮秒内出现，并呈现出明显变宽的类似脱氧血红蛋白的光谱，该光谱持续到 680 皮秒。光谱的改变归因于强烈激发可能导致的脱氧血红素构象和自旋态的产生。
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