表面膜提取物对3T3细胞生长的抑制作用。
Inhibition of growth of 3T3 cells by extract of surface membranes.
作者信息
Whittenberger B, Raben D, Lieberman M A, Glaser L
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5457-61. doi: 10.1073/pnas.75.11.5457.
Abstract
The growth of Swiss 3T3 cells can be inhibited by the addition of a plasma membrane enriched fraction from 3T3 cells [Whittenberger, B & Glaser, L. (1977) Proc. Natl. Acad. Sci. USA 74, 2251--2255]. We report conditions for the extraction of the inhibitory components from the membranes with octylglucoside. The solubilized components reversibly inhibit the rate of cell division of 3T3 but not of simian virus 40-transformed 3T3 cells. The inhibitory activity is heat labile and nondialyzable. The inhibition observed with the soluble extract can be reversed by mitogenic factors present in serum.
摘要
添加来自3T3细胞的富含质膜的组分能够抑制瑞士3T3细胞的生长[惠滕伯格，B和格拉泽，L.（1977年）《美国国家科学院院刊》74，2251 - 2255]。我们报告了用辛基葡糖苷从膜中提取抑制成分的条件。溶解的成分可逆地抑制3T3细胞的细胞分裂速率，但不抑制猿猴病毒40转化的3T3细胞的细胞分裂速率。抑制活性对热不稳定且不可透析。血清中存在的促有丝分裂因子可逆转可溶性提取物所观察到的抑制作用。
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本文引用的文献
1
TRANSFORMATION OF PROPERTIES OF AN ESTABLISHED CELL LINE BY SV40 AND POLYOMA VIRUS.SV40和多瘤病毒对已建细胞系特性的转化
Proc Natl Acad Sci U S A. 1964 Jan;51(1):66-73. doi: 10.1073/pnas.51.1.66.
2
Conditions determining initiation of DNA synthesis in 3T3 cells.决定3T3细胞中DNA合成起始的条件。
Proc Natl Acad Sci U S A. 1970 May;66(1):204-10. doi: 10.1073/pnas.66.1.204.
3
"Contact inhibition" of cell division in 3T3 cells.3T3细胞中细胞分裂的“接触抑制”
Proc Natl Acad Sci U S A. 1968 May;60(1):300-4. doi: 10.1073/pnas.60.1.300.
4
Shaking 3T3 cells: further studies on diffusion boundary effects.摇晃3T3细胞：关于扩散边界效应的进一步研究。
Cell. 1974 Nov;3(3):207-15. doi: 10.1016/0092-8674(74)90133-0.
5
A restriction point for control of normal animal cell proliferation.正常动物细胞增殖控制的一个限制点。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1286-90. doi: 10.1073/pnas.71.4.1286.
6
Kinetics of early changes in phosphate and uridine transport and cyclic AMP levels stimulated by serum in density-inhibited 3T3 cells.血清刺激下密度抑制的3T3细胞中磷酸盐和尿苷转运以及环磷酸腺苷水平早期变化的动力学
Proc Natl Acad Sci U S A. 1974 Jan;71(1):96-8. doi: 10.1073/pnas.71.1.96.
7
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8
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10
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Proc Natl Acad Sci U S A. 1976 Feb;73(2):396-400. doi: 10.1073/pnas.73.2.396.
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