分离的表面膜对3T3细胞培养物中DNA合成的抑制作用。
Inhibition of DNA synthesis in cultures of 3T3 cells by isolated surface membranes.
作者信息
Whittenberger B, Glaser L
出版信息
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2251-5. doi: 10.1073/pnas.74.6.2251.
Abstract
When added to a sparse culture of 3T3 cells, a surface membrane-enriched fraction from 3T3 cells inhibited the rate of DNA synthesis in a time- and concentration-dependent manner. The membrane preparation had no effect on the rate of DNA synthesis of simian virus 40-transformed 3T3 cells. A similar membrane preparation from transformed cells had a lesser inhibitory effect on 3T3 cells and no effect on transformed cells. The inhibition by membranes was reversible. The data suggest that, when added to growing 3T3 cells, 3T3 surface membranes can mimic the effect of increasing cell density on DNA synthesis.
摘要
当将来自3T3细胞的富含表面膜的组分添加到3T3细胞的稀疏培养物中时，其以时间和浓度依赖性方式抑制DNA合成速率。该膜制剂对猿猴病毒40转化的3T3细胞的DNA合成速率没有影响。来自转化细胞的类似膜制剂对3T3细胞的抑制作用较小，对转化细胞没有影响。膜的抑制作用是可逆的。数据表明，当添加到生长的3T3细胞中时，3T3表面膜可以模拟细胞密度增加对DNA合成的影响。
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