de Souza Pereira Jorge J, Pereira Aline de P C, Jandú Jannyson J B, da Paz Josinete A, Crovella Sergio, Dos Santos Correia Maria T, de Azevêdo Silva Jaqueline
Department of Genetics, Federal University of PernambucoRecife, Brazil; Laboratory of Immunopathology Keizo Asami, Federal University of PernambucoRecife, Brazil.
Laboratory of Glycoproteins, Department of Biochemistry, Federal University of Pernambuco Recife, Brazil.
Front Microbiol. 2017 Jan 24;8:52. doi: 10.3389/fmicb.2017.00052. eCollection 2017.
is a plant specie usually known for its medicinal purposes in local communities in Northeast Brazil. In order to evaluate its therapeutic potential, we aimed to determine the phytochemical and antimicrobial properties of extracts. Thin Layer Chromatography (TLC) was able to detect the presence of phenolic compounds, flavonoids and reducing sugars. Three phenolic compounds were identified by HPLC and described as Gallic, Chlorogenic and Protocatechuic acids. On the other hand, HNMR analysis revealed the presence of hinokinin, a bioactive lignan further characterized in the present work. The minimum inhibitory concentration (MIC) values for hinokinin ranged from 0.0485 to 3.125 mg/mL in different clinical isolates and showed a bactericidal activity against MRSA isolated from blood (MMC 0.40 mg/mL) and postoperative secretion (MMC = 3.125 mg/mL). extracts also showed antimicrobial activity against Mycobacterium species such as (MIC = 12.5 mg/mL) and (MIC = 52 mg/mL). Additionally, we determined the toxicity of by HC tests with hemolytic activity detected of 313 ± 0.5 μg/mL. Our results showed that possesses inhibitory properties against MRSA as well as several other clinically important microorganisms. Furthermore, the present work is the first report of the presence of hinokinin in Commiphora genus.
是一种在巴西东北部当地社区通常因其药用价值而闻名的植物物种。为了评估其治疗潜力，我们旨在确定提取物的植物化学和抗菌特性。薄层色谱法（TLC）能够检测到酚类化合物、黄酮类化合物和还原糖的存在。通过高效液相色谱法（HPLC）鉴定出三种酚类化合物，分别为没食子酸、绿原酸和原儿茶酸。另一方面，核磁共振氢谱（HNMR）分析显示存在扁柏素，这是一种在本研究中进一步表征的生物活性木脂素。扁柏素在不同临床分离株中的最低抑菌浓度（MIC）值范围为0.0485至3.125 mg/mL，并对从血液中分离出的耐甲氧西林金黄色葡萄球菌（MMC 0.40 mg/mL）和术后分泌物（MMC = 3.125 mg/mL）表现出杀菌活性。提取物对结核分枝杆菌属物种如（MIC = 12.5 mg/mL）和（MIC = 52 mg/mL）也显示出抗菌活性。此外，我们通过溶血活性检测为313±0.5 μg/mL的HC试验确定了的毒性。我们的结果表明对耐甲氧西林金黄色葡萄球菌以及其他几种临床上重要的微生物具有抑制特性。此外，本研究是首次报道没药属中存在扁柏素。
