Zhao Tao, Ma Fenglian, Yin Fangqing
Department of Pediatric Surgery, Linyi Peoples' Hospital, Linyi, China.
Department of Pediatric Surgery, Linyi Peoples' Hospital, Linyi, China -
Minerva Pediatr. 2018 Apr;70(2):185-196. doi: 10.23736/S0026-4946.17.04657-6. Epub 2017 Feb 7.
The association between glutathione-S-transferase polymorphisms (GSTM1, GSTT1 and GSTP1 Ile105Val) and risk of childhood acute lymphoblastic leukemia (ALL) remains controversial. Therefore, we performed a meta-analysis to derive a more precise estimation of the relationship.
We searched Medline (Mainly Pubmed), Embase, Cochrane Library, CNKI (Chinese National Knowledge Infrastructure), Chinese Biomedical Literature (Chinese) and Wanfang (Chinese) Databases to collect articles that evaluated the polymorphism of GSTs gene in child patients with ALL. We also reviewed reference lists from retrieved articles. Two researchers evaluated study eligibility and extracted the data independently, and disagreements were resolved by discussion. Two reviewers independently assessed the methodological quality of each study. The principal outcome measure was the odds ratio (OR) with 95% confidence interval (CI) for the risk of child ALL associated with GSTM1, GSTT1, GSTP1 Ile105Val. The data were analyzed using Stata (Version 12.0) software.
This meta-analysis included 30 studies for GSTM1 polymorphism, 27 studies for GSTM1 polymorphism and 10 studies for GSTP1 Ile105Val polymorphism. The combined results based on all studies showed that GSTM1 (OR= 1.297, 95% CI= 1.105-1.523, P=0.001) and GSTT1 polymorphism (OR=1.213, 95% CI=1.031-1.427, P= 020) contributes to the development of childhood ALL, while no association was found in all comparison models of GSTP1 Ile105Val variants (homozygote model: OR=1.234, 95% CI=0.966-1.578, P=0.093, heterozygote model: OR=1.072, 95% CI=0.931-1.235, P=0.331, dominant model: OR=1.104, 95% CI=0.965-1.262, P=0.149, recessive model: OR=1.184, 95% CI=0.934-1.499, P=0.162, and allele comparison model: OR=1.096, 95% CI=0.988-1.216, P=0.083).
The current analyses suggests significant association was found between GSTM1 variants and the risk of childhood ALL, while no association were found between GSTT1 and GSTP1 Ile105Val polymorphism and childhood ALL risk.
谷胱甘肽-S-转移酶基因多态性（GSTM1、GSTT1和GSTP1 Ile105Val）与儿童急性淋巴细胞白血病（ALL）风险之间的关联仍存在争议。因此，我们进行了一项荟萃分析，以更精确地评估二者之间的关系。
我们检索了医学文献数据库（主要是PubMed）、Embase、Cochrane图书馆、中国知网（CNKI）、中国生物医学文献数据库（中文）和万方数据库（中文），以收集评估ALL儿童患者GSTs基因多态性的文章。我们还查阅了检索文章的参考文献列表。两名研究人员独立评估研究的合格性并提取数据，如有分歧通过讨论解决。两名评审员独立评估每项研究的方法学质量。主要结局指标是与GSTM1、GSTT1、GSTP1 Ile105Val相关的儿童ALL风险的比值比（OR）及95%置信区间（CI）。使用Stata（12.0版）软件对数据进行分析。
这项荟萃分析包括30项关于GSTM1多态性的研究、27项关于GSTT1多态性的研究和10项关于GSTP1 Ile105Val多态性的研究。基于所有研究的综合结果显示，GSTM1（OR = 1.297，95%CI = 1.105 - 1.523，P = 0.001）和GSTT1多态性（OR = 1.213，95%CI = 1.031 - 1.427，P = 0.020）与儿童ALL的发生有关，而在GSTP1 Ile105Val变体的所有比较模型中均未发现关联（纯合子模型：OR = 1.234，95%CI = 0.966 - 1.578，P = 0.093；杂合子模型：OR = 1.072，95%CI = 0.931 - 1.235，P = 0.331；显性模型：OR = 1.104，95%CI = 0.965 - 1.262，P = 0.149；隐性模型：OR = 1.184，95%CI = 0.934 - 1.499，P = 0.162；等位基因比较模型：OR = 1.096，9(5%CI = 0.988 - 1.216，P = 0.083）。
当前分析表明，GSTM1变体与儿童ALL风险之间存在显著关联，而GSTT1和GSTP1 Ile105Val多态性与儿童ALL风险之间未发现关联。
