Ağababaoğlu İsmail, Önen Ahmet, Demir Ayşe Banu, Aktaş Safiye, Altun Zekiye, Ersöz Hasan, Şanl Aydn, Özdemir Nezih, Akkoçlu Atila
Department of Thoracic Surgery, Dokuz Eylül University Department of Medical Biology, Izmir University of Economics Faculty of Medicine Department of Basic Oncology, Dokuz Eylül University Institute of Oncology Department of Chest Diseases, Dokuz Eylül University Medicine School, İzmir, Turkey.
Medicine (Baltimore). 2017 Feb;96(6):e5903. doi: 10.1097/MD.0000000000005903.
Lung cancer is responsible of 12.4% and 17.6% of all newly diagnosed cancer cases and mortality due to cancer, respectively, and 5-year survival rate despite all improved treatment options is 15%. This survival rate reaches 66% in the Stage 1 and surgically treated patients. Early diagnosis which could not be definitely and commonly achieved yet is extremely critical in obtaining high survival rate in this disease. For this reason; proteomic differences were evaluated using matrix assisted laser desorption ionization (MALDI) mass spectrometry in the subgroups of lung adenocarcinoma and squamous cell carcinoma.
Fresh tissue samples of 36 malignant cases involving 83.3% (n = 30) men and 16.7% (n = 6) women patients were distributed into 2 groups as early and end stage lung cancer and each group were composed of subgroups including 18 squamous cell carcinoma (9 early stage cases, 9 end stage cases) and 18 adenocarcinoma cases (9 early stage cases, 9 end stage cases). The fresh tissues obtained from the tumoral and matched normal sites after surgical intervention. The differences in protein expression levels were determined by comparing proteomic changes in each patient.
In the subgroups of advanced stage adenocarcinoma; tumoral tissue revealed differences in expression of 2 proteins compared with normal parenchymal tissue. Of those; difference in protein expression in heat shock protein 60 (HSP60) was found statistically significant (P = 0.0001). Subgroups of early and advanced stage squamos cell carcinoma have differed in certain 20 protein expression of normal tissue and diseased squamos cell carcinoma. Of those, increased protein expression level of only annexin-2 protein was found statistically significant (P = 0.002). No significant difference was detected in early and advanced stage protein expressions of the tumoral tissues in the subgroups of adenocarcinoma and squamous cell carcinoma.
We conclude that with respect to early diagnosis of lung cancer that HSP60 and annexin-2 proteins are the important biomarkers in the subgroups of adenocarcinoma and squamous cell carcinoma. We also consider that these 2 proteins are molecules which may provide critical contribution in evaluation of prognosis, metastatic potential, response to treatment, and in establishment of differential diagnosis between adenocarcinoma and squamous cell carcinoma.
肺癌分别占所有新诊断癌症病例的12.4%和癌症死亡病例的17.6%，尽管有各种改进的治疗方案，其5年生存率仍为15%。在I期接受手术治疗的患者中，这一生存率可达66%。目前尚无法普遍实现的早期诊断对于提高该疾病的生存率极为关键。因此，使用基质辅助激光解吸电离（MALDI）质谱法评估了肺腺癌和鳞状细胞癌亚组中的蛋白质组差异。
36例恶性病例的新鲜组织样本，其中男性患者占83.3%（n = 30），女性患者占16.7%（n = 6），分为早期和晚期肺癌两组，每组又由18例鳞状细胞癌（9例早期病例，9例晚期病例）和18例腺癌病例（9例早期病例，9例晚期病例）组成。手术干预后从肿瘤部位和匹配的正常部位获取新鲜组织。通过比较每位患者的蛋白质组变化来确定蛋白质表达水平的差异。
在晚期腺癌亚组中，肿瘤组织与正常实质组织相比，有2种蛋白质的表达存在差异。其中，热休克蛋白60（HSP60）的蛋白质表达差异具有统计学意义（P = 0.0001）。早期和晚期鳞状细胞癌亚组在正常组织和患病鳞状细胞癌的某些20种蛋白质表达上存在差异。其中，仅膜联蛋白-2蛋白的蛋白质表达水平升高具有统计学意义（P = 0.002）。腺癌和鳞状细胞癌亚组中肿瘤组织的早期和晚期蛋白质表达未检测到显著差异。
我们得出结论，就肺癌的早期诊断而言，HSP60和膜联蛋白-2蛋白是腺癌和鳞状细胞癌亚组中的重要生物标志物。我们还认为这两种蛋白质可能在评估预后、转移潜能、治疗反应以及建立腺癌和鳞状细胞癌的鉴别诊断方面发挥关键作用。
