Department of Marine Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Department of Biomedical Science and Environmental Biology, KMU - Kaohsiung Medical University, No. 100, Shin-Chuan 1st Road, Kaohsiung 80708, Taiwan, ROC; Department of Marine Biotechnology and Resources, National Sun Yat-sen University, No. 70, Lienhai Road, Kaohsiung 80424, Taiwan, ROC.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:743-755. doi: 10.1016/j.msec.2016.12.062. Epub 2016 Dec 15.
Eight metal and non-metal coupons were exposed to seawater in offshore platform laboratory on biofilm formation in seven days of every month interval time for one year period. Among metal coupons, the highest mean heterotrophic biofilm bacteria population (HBBP) ranges were observed in mild steel (MS) coupon (33.03 to 40.00 [×10] CFUscm) and lowest in copper (Cu) coupon (3.0 to 5.1 [×10] CFUscm). Non-metal coupons show, highest mean HBBP ranges in glass (GL) coupon (29.6 to 66.0 [×10] CFUscm) and lowest in polyvinyl chloride (PVC) coupon (34.4 to 52.5 [×10] CFUscm). Within the studied coupons, high HBBP level was witnessed in GL and simultaneously low in Cu due to its toxic leaching product of CuO. Gram-positive bacterial deposit show dominated trend in all coupons with increasing order of Cu>SS>MS>PVC>GL>Cu-Ni 70/30>Cu-Ni 90/10>Br and Gram-negative show different in the order of Br>Cu-Ni 90/10>Cu-Ni 70/30>GL>PVC>MS>SS>Cu, respectively. The 16S rDNA sequence confirmed, heterotrophic biofilm Bacillus sp. (Gram-positive) were dominated in all coupons for all months.
在为期一年的时间里，每隔七天，将 8 个金属和非金属试片暴露在海上平台实验室的海水中，以观察其在 7 天时间内的生物膜形成情况。在金属试片中，中碳钢（MS）试片上的异养生物膜细菌种群（HBBP）平均值最高（33.03 至 40.00 [×10] CFUscm），而铜试片上的 HBBP 平均值最低（3.0 至 5.1 [×10] CFUscm）。而非金属试片中，玻璃（GL）试片上的 HBBP 平均值最高（29.6 至 66.0 [×10] CFUscm），聚氯乙烯（PVC）试片上的 HBBP 平均值最低（34.4 至 52.5 [×10] CFUscm）。在所研究的试片中，由于氧化铜的有毒浸出物，GL 试片的 HBBP 水平较高，而同时 Cu 试片的 HBBP 水平较低。所有试片中都以革兰氏阳性菌为主，依次为 Cu>SS>MS>PVC>GL>Cu-Ni 70/30>Cu-Ni 90/10>Br，而革兰氏阴性菌的顺序则不同，依次为 Br>Cu-Ni 90/10>Cu-Ni 70/30>GL>PVC>MS>SS>Cu。16S rDNA 序列证实，所有月份的所有试片中，异养生物膜 Bacillus sp.（革兰氏阳性菌）均占主导地位。
