Kim Jung Ok, Song Sae Am, Yoon Eun-Jeong, Shin Jeong Hwan, Lee Hyukmin, Jeong Seok Hoon, Lee Kyungwon
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea.
Department of Laboratory Medicine, Inje University College of Medicine, Busan, South Korea.
Diagn Microbiol Infect Dis. 2017 Apr;87(4):343-348. doi: 10.1016/j.diagmicrobio.2016.12.012. Epub 2016 Dec 27.
Over a 5-month period between the end of June and the beginning of November in 2015, a KPC-producing Enterobacteriaceae outbreak occurred in a general hospital in Busan, South Korea, being associated with a total of 50 clinical isolates from 47 patients. Multilocus sequence typing and pulsed-field gel electrophoresis were carried out for strain typing and whole-genome sequencing was performed to characterize the plasmids. A clonal spread of K. pneumoniae sequence type 307 (ST307) carrying a self-transferable IncX3-type plasmid harboring bla was responsible for the outbreak. Sporadic emergence of K. pneumoniae ST697 carrying an IncFII-type plasmid and a ST11 isolate harboring a small plasmid devoid of any known origin of replication were observed to be associated with bla, but no further dissemination of these strains was identified. The results indicated a healthcare-associated infection associated with a bla-harboring plasmid dissemination and a clonal spread of KPC-producing Enterobacteriaceae.
在2015年6月底至11月初的5个月期间，韩国釜山一家综合医院发生了产KPC的肠杆菌科细菌暴发，共有来自47名患者的50株临床分离株与之相关。采用多位点序列分型和脉冲场凝胶电泳进行菌株分型，并进行全基因组测序以表征质粒。携带bla的可自我转移的IncX3型质粒的肺炎克雷伯菌序列型307（ST307）的克隆传播是此次暴发的原因。观察到携带IncFII型质粒的肺炎克雷伯菌ST697和携带无任何已知复制起点的小质粒的ST11分离株的零星出现与bla相关，但未发现这些菌株的进一步传播。结果表明这是一次与携带bla的质粒传播及产KPC肠杆菌科细菌的克隆传播相关的医疗保健相关感染。
