Lin Zhideng, Wang Xiaodan, Bu Xianyong, Huang Qincheng, Wang Han, Li Erchao, Qin Jianguang, Chen Liqiao
Laboratory of Aquaculture Nutrition and Environmental Health, College of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
College of Marine Science, Ningde Normal University, Fujian 352100, China.
Aquac Nutr. 2025 Feb 12;2025:5627355. doi: 10.1155/anu/5627355. eCollection 2025.
The present study investigates the effects of dietary phosphatidylcholine (PC) deficiency and its addition on growth and physiological and biochemical indicators of juvenile under different oil sources. There were no significant differences in the growth and feed utilization between the vegetable oils and fish oil (FO) groups under PC-devoid conditions. In contrast, the FO and perilla oil (PO) groups showed better growth-promoting effects and higher feed utilization than the safflower oil (SO) and olive oil (OO) groups under 3% PC-added condition. Both dietary PC and oil sources (FO or PO) could inhibit lipid accumulation of the whole crab, and dietary PC also observably facilitated whole-body protein deposition. In addition, dietary FO and PO increased the burden of the antioxidant system and the risk of lipid peroxidation in juvenile . Meanwhile, diets supplemented with PC effectively alleviated oxidative stress and lipid peroxidation caused by dietary FO and PO. The composition of fatty acids in muscle and hepatopancreas was positively associated with that in diets. Compared with SO and OO, FO and PO significantly reduced the lipid deposition in the hepatopancreas at 3% PC supplementation, possibly because FO and PO formed new physiological-active PC contained n-3 polyunsaturated fatty acids (PUFAs) with dietary PC through activating PC remodeling reaction, and promoting fatty acid utilization, and finally inhibiting the lipid accumulation in the hepatopancreas. This study indicates that FO and PO are better lipid sources (LSs) for , providing alternative oil sources in the crab diet in combination with PC supplementation.
本研究调查了膳食磷脂酰胆碱（PC）缺乏及其添加对不同油源下幼蟹生长及生理生化指标的影响。在缺乏PC的条件下，植物油组和鱼油（FO）组在生长和饲料利用率方面无显著差异。相比之下，在添加3%PC的条件下，FO组和紫苏油（PO）组比红花油（SO）组和橄榄油（OO）组表现出更好的促生长效果和更高的饲料利用率。膳食PC和油源（FO或PO）均可抑制幼蟹整体脂质积累，膳食PC还显著促进了全身蛋白质沉积。此外，膳食FO和PO增加了幼蟹抗氧化系统的负担以及脂质过氧化风险。同时，补充PC的日粮有效缓解了膳食FO和PO引起的氧化应激和脂质过氧化。肌肉和肝胰腺中的脂肪酸组成与日粮中的脂肪酸组成呈正相关。与SO和OO相比，在补充3%PC时，FO和PO显著降低了肝胰腺中的脂质沉积，这可能是因为FO和PO通过激活PC重塑反应与膳食PC形成了含有n-3多不饱和脂肪酸（PUFA）的新的生理活性PC，促进了脂肪酸利用，最终抑制了肝胰腺中的脂质积累。本研究表明，FO和PO是幼蟹更好的脂质来源，在蟹类日粮中与补充PC相结合可提供替代油源。
