Lorenzen Emma, Follmann Frank, Secher Jan O, Goericke-Pesch Sandra, Hansen Mette S, Zakariassen Hannah, Olsen Anja W, Andersen Peter, Jungersen Gregers, Agerholm Jørgen S
Section for Veterinary Reproduction and Obstetrics, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Dyrlægevej 68, 1870, Frederiksberg C, Denmark; Chlamydia Vaccine Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Artillerivej 5, 2300, København S, Denmark.
Chlamydia Vaccine Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Artillerivej 5, 2300, København S, Denmark.
Microbes Infect. 2017 Jun;19(6):334-342. doi: 10.1016/j.micinf.2017.01.008. Epub 2017 Feb 9.
Advanced animal models, such as minipigs, are needed for the development of a globally requested human Chlamydia vaccine. Previous studies have shown that vaginal inoculation of sexually mature Göttingen minipigs with Chlamydia trachomatis resulted in an infection lasting only 3-5 days. The aim of this study was to evaluate the effect of targeting the upper porcine genital tract by transcervical and transabdominal intrauterine inoculation, compared to previously performed vaginal inoculation. Furthermore, we investigated the effect of the hormonal cycle, estrus vs. diestrus, on the establishment of a C. trachomatis infection in the minipig. Targeting the upper genital tract (transcervical inoculation) resulted in a longer lasting infection (at least 7 days) compared to vaginal inoculation (3-5 days). When comparing intrauterine inoculation during estrus and diestrus, inoculation during diestrus resulted in a longer lasting infection (at least 10 days) compared to estrus (3-5 days). Furthermore, we found a significant C. trachomatis specific IFN-γ response in pigs inoculated during estrus correlating with the accelerated clearance of infection in these pigs. These findings suggest that for implementation of an optimal model of C. trachomatis in minipigs, inoculation should bypass the cervix and preferable be performed during diestrus.
开发全球所需的人类衣原体疫苗需要先进的动物模型，如小型猪。先前的研究表明，对性成熟的哥廷根小型猪进行阴道接种沙眼衣原体，感染仅持续3至5天。本研究的目的是评估经宫颈和经腹子宫内接种靶向猪上生殖道的效果，并与先前进行的阴道接种进行比较。此外，我们研究了激素周期（发情期与黄体期）对小型猪沙眼衣原体感染建立的影响。与阴道接种（3至5天）相比，靶向于上生殖道（经宫颈接种）导致感染持续时间更长（至少7天）。比较发情期和黄体期的子宫内接种时，黄体期接种导致的感染持续时间比发情期（3至5天）更长（至少10天）。此外，我们发现在发情期接种的猪中存在显著的沙眼衣原体特异性干扰素-γ反应，这与这些猪中感染的加速清除相关。这些发现表明，为了在小型猪中建立最佳的沙眼衣原体模型，接种应绕过宫颈，并且最好在黄体期进行。
