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用CAF01佐剂的紫外线灭活细菌进行肌肉注射免疫的小型猪中，CD4和CD8 T淋巴细胞、IgA和IgG浆细胞的生殖器浸润以及粘膜内淋巴滤泡与预防生殖器感染相关。

Genital Infiltrations of CD4 and CD8 T Lymphocytes, IgA and IgG Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01.

作者信息

Erneholm Karin, Lorenzen Emma, Bøje Sarah, Olsen Anja Weinreich, Jungersen Gregers, Jensen Henrik E, Cassidy Joseph P, Andersen Peter, Agerholm Jørgen S, Follmann Frank

机构信息

Section of Veterinary Reproduction and Obstetrics, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.

Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark.

出版信息

Front Microbiol. 2019 Feb 8;10:197. doi: 10.3389/fmicb.2019.00197. eCollection 2019.

DOI:10.3389/fmicb.2019.00197
PMID:30800114
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6375829/
Abstract

The development of a vaccine against genital chlamydia in women is advancing, and the evaluation of immune responses following vaccination and challenge infections is crucial for development of a safe and protective vaccine. This study employs the sexually mature minipig model to characterize the genital immune response to infection in pigs previously immunized intramuscularly with UV-inactivated serovar D (UV-SvD) adjuvanted/formulated with CAF01 adjuvant compared to a CAF01-alone control group. Pigs immunized with UV-SvD were significantly protected against vaginal challenge with on day 3 post inoculation and showed significantly higher cervical infiltrations of approximately equal numbers of CD4 and CD8 T-cells, and IgG and IgA plasma cells compared to adjuvant-alone immunized controls. These immunological signatures correspond to findings in mice and are similar to those described in female chlamydia patients. This proves important potential for the pig model in elucidating immunological signatures in future translational research in chlamydia vaccinology.

摘要

针对女性生殖系统衣原体的疫苗研发正在推进，接种疫苗及感染激发后的免疫反应评估对于安全有效的疫苗开发至关重要。本研究采用性成熟小型猪模型，以表征先前经肌肉注射用CAF01佐剂佐剂化/配制的紫外线灭活血清型D（UV-SvD）免疫的猪，相较于单独使用CAF01的对照组，其对感染的生殖系统免疫反应。接种UV-SvD的猪在接种后第3天对阴道激发具有显著的保护作用，并且与单独接种佐剂的对照组相比，其宫颈中CD4和CD8 T细胞数量大致相等，IgG和IgA浆细胞浸润显著更高。这些免疫特征与小鼠研究结果相符，且与衣原体感染女性患者中所描述的特征相似。这证明了猪模型在衣原体疫苗学未来转化研究中阐明免疫特征方面具有重要的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/7bd0d8f52d3c/fmicb-10-00197-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/90005a081a1e/fmicb-10-00197-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/c521ee6e507a/fmicb-10-00197-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/4d2dd99c1e60/fmicb-10-00197-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/7bd0d8f52d3c/fmicb-10-00197-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/90005a081a1e/fmicb-10-00197-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/c521ee6e507a/fmicb-10-00197-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/4d2dd99c1e60/fmicb-10-00197-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa06/6375829/7bd0d8f52d3c/fmicb-10-00197-g004.jpg

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Genital Infiltrations of CD4 and CD8 T Lymphocytes, IgA and IgG Plasma Cells and Intra-Mucosal Lymphoid Follicles Associate With Protection Against Genital Infection in Minipigs Intramuscularly Immunized With UV-Inactivated Bacteria Adjuvanted With CAF01.用CAF01佐剂的紫外线灭活细菌进行肌肉注射免疫的小型猪中，CD4和CD8 T淋巴细胞、IgA和IgG浆细胞的生殖器浸润以及粘膜内淋巴滤泡与预防生殖器感染相关。
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Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus.
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