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急性-on-慢性肝衰竭中调节性T细胞的失调：大鼠模型

Deregulation of Regulatory T Cells in Acute-on-Chronic Liver Failure: A Rat Model.

作者信息

Ni Shunlan, Li Shanshan, Yang Naibin, Tang Xinyue, Zhang Shengguo, Hu Danping, Lu Mingqin

机构信息

Department of Infectious Diseases, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, China.

Department of Infectious Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou Key Laboratory of Hepatology, Hepatology Institute of Wenzhou Medical University, Wenzhou, Zhejiang, China.

出版信息

Mediators Inflamm. 2017;2017:1390458. doi: 10.1155/2017/1390458. Epub 2017 Jan 15.

DOI:10.1155/2017/1390458
PMID:28194045
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5282067/
Abstract

. Acute-on-chronic liver failure (ACLF) and acute liver failure (ALF) are similar in many respects during their acute exacerbation; however, ACLF generally has a poorer prognosis. We aimed to investigate the role and dynamic changes of regulatory T cell (Treg) and T helper 17 (Th17) cell proportions during ACLF progress. . All rats were classified into two groups randomly: ACLF group and ALF group (control group). The rat model of ACLF was preestablished by intraperitoneal injection of carbon tetrachloride for 2 months. Then acute liver injury was induced by combined D-galactosamine and lipopolysaccharide. Six time points were examined before or after acute induction. Liver samples were performed with hematoxylin-eosin and Masson staining; circulatory Treg and Th17 cell frequencies were determined using flow cytometry assays; serum levels of alanine aminotransferase, aspartate aminotransferase, interleukin-10 (IL-10), and interferon- (IFN-) were examined. . In group ACLF, both Th17 cell proportion and IFN- level presented upgrade firstly and then descend latter tendency; the trends of Treg cell proportion and IL-10 level were observed to gradually decrease and became stable. . The Treg cells played an important role in the immunologic mechanism during the process of ACLF. And the function of Treg cells in ACLF was defective.

摘要

急性慢性肝衰竭（ACLF）和急性肝衰竭（ALF）在急性加重期的许多方面相似；然而，ACLF的预后通常较差。我们旨在研究调节性T细胞（Treg）和辅助性T细胞17（Th17）细胞比例在ACLF进展过程中的作用及动态变化。

所有大鼠随机分为两组

ACLF组和ALF组（对照组）。通过腹腔注射四氯化碳2个月建立ACLF大鼠模型。然后用D-半乳糖胺和脂多糖联合诱导急性肝损伤。在急性诱导前后检查六个时间点。肝组织样本进行苏木精-伊红和Masson染色；使用流式细胞术测定循环Treg和Th17细胞频率；检测血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）水平。

在ACLF组中，Th17细胞比例和IFN-γ水平均呈现先升高后下降的趋势；Treg细胞比例和IL-10水平则逐渐降低并趋于稳定。

Treg细胞在ACLF过程的免疫机制中起重要作用。并且ACLF中Treg细胞的功能存在缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/8d63f0b61e44/MI2017-1390458.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/8097b0225adf/MI2017-1390458.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/9c9a9ee1795c/MI2017-1390458.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/5c360bf355cb/MI2017-1390458.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/7396b7c1f127/MI2017-1390458.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/1e88bc49f3bf/MI2017-1390458.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/d7c166e059f5/MI2017-1390458.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/8d63f0b61e44/MI2017-1390458.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/8097b0225adf/MI2017-1390458.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/9c9a9ee1795c/MI2017-1390458.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/5c360bf355cb/MI2017-1390458.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/7396b7c1f127/MI2017-1390458.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/1e88bc49f3bf/MI2017-1390458.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/d7c166e059f5/MI2017-1390458.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c396/5282067/8d63f0b61e44/MI2017-1390458.007.jpg

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