Song Zhenqiao, Guo Linlin, Liu Tian, Lin Caicai, Wang Jianhua, Li Xingfeng
Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China; State Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Agronomy College, Shandong Agricultural University, Tai'an, Shandong 271028, China.
Int J Genomics. 2017;2017:9364594. doi: 10.1155/2017/9364594. Epub 2017 Jan 17.
Bunge is an important traditional Chinese medicine (TCM). In this study, two genotypes (BH18 and ZH23) with different phenolic acid concentrations were used for de novo RNA sequencing (RNA-seq). A total of 170,787 transcripts and 56,216 unigenes were obtained. There were 670 differentially expressed genes (DEGs) identified between BH18 and ZH23, 250 of which were upregulated in ZH23, with genes involved in the phenylpropanoid biosynthesis pathway being the most upregulated genes. Nine genes involved in the lignin biosynthesis pathway were upregulated in BH18 and thus result in higher lignin content in BH18. However, expression profiles of most genes involved in the core common upstream phenylpropanoid biosynthesis pathway were higher in ZH23 than that in BH18. These results indicated that genes involved in the core common upstream phenylpropanoid biosynthesis pathway might play an important role in downstream secondary metabolism and demonstrated that lignin biosynthesis was a putative partially competing pathway with phenolic acid biosynthesis. The results of this study expanded our understanding of the regulation of phenolic acid biosynthesis in
地锦是一种重要的传统中药。在本研究中，使用了两种酚酸浓度不同的基因型（BH18和ZH23）进行从头RNA测序（RNA-seq）。共获得170,787个转录本和56,216个单基因。在BH18和ZH23之间鉴定出670个差异表达基因（DEG），其中250个在ZH23中上调，参与苯丙烷生物合成途径的基因是上调最多的基因。9个参与木质素生物合成途径的基因在BH18中上调，因此导致BH18中木质素含量更高。然而，参与核心共同上游苯丙烷生物合成途径的大多数基因的表达谱在ZH23中高于BH18。这些结果表明，参与核心共同上游苯丙烷生物合成途径的基因可能在下游次生代谢中起重要作用，并表明木质素生物合成是与酚酸生物合成的一种假定的部分竞争途径。本研究结果扩展了我们对酚酸生物合成调控的理解
