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干旱胁迫下的转录组分析

Transcriptome Analysis of under Drought Stress.

作者信息

Zhang Siwei, Qi Xinlan, Zhu Ruiyan, Ye Dongdong, Shou Minyu, Peng Lulu, Qiu Minghua, Shi Min, Kai Guoyin

机构信息

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China.

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

出版信息

Plants (Basel). 2024 Jan 6;13(2):161. doi: 10.3390/plants13020161.

DOI:10.3390/plants13020161
PMID:38256715
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10819027/
Abstract

Phenolic acids are one of the major secondary metabolites accumulated in with various pharmacological activities. Moderate drought stress can promote the accumulation of phenolic acids in , while the mechanism remains unclear. Therefore, we performed transcriptome sequencing of under drought treatment. A total of 47,169 unigenes were successfully annotated in at least one of the six major databases. Key enzyme genes involved in the phenolic acid biosynthetic pathway, including , , , , , and , were induced. Unigenes annotated as laccase correlated with S and were analyzed, and seven candidates that may be involved in the key step of SalB biosynthesis by RA were obtained. A total of 15 transcription factors significantly up-regulated at 2 h and 4 h potentially regulating phenolic acid biosynthesis were screened out. TRINITY_DN14213_c0_g1 (AP2/ERF) significantly transactivated the expression of and , suggesting its role in the regulation of phenolic acid biosynthesis. GO and KEGG enrichment analysis of differential expression genes showed that phenylpropanoid biosynthesis and plant hormone signal transduction were significantly higher. The ABA-dependent pathway is essential for resistance to drought and phenolic acid accumulation. Expression patterns in drought and ABA databases showed that four PYLs respond to both drought and ABA, and three potential SnRK2 family members were annotated and analyzed. The present study presented a comprehensive transcriptome analysis of affected by drought, which provides a rich source for understanding the molecular mechanism facing abiotic stress in .

摘要

酚酸是积累的主要次生代谢产物之一，具有多种药理活性。适度干旱胁迫可促进酚酸在[具体植物名称未给出]中的积累，但其机制尚不清楚。因此，我们对干旱处理下的[具体植物名称未给出]进行了转录组测序。共有47169个单基因在六个主要数据库中的至少一个中成功注释。参与酚酸生物合成途径的关键酶基因，包括[具体基因名称未给出]等被诱导。对注释为与S和[具体物质未给出]相关的漆酶的单基因进行了分析，获得了七个可能参与RA合成SalB关键步骤的候选基因。共筛选出15个在2小时和4小时显著上调、可能调控酚酸生物合成的转录因子。TRINITY_DN14213_c0_g1（AP2/ERF）显著激活了[具体基因名称未给出]和[具体基因名称未给出]的表达，表明其在酚酸生物合成调控中的作用。差异表达基因的GO和KEGG富集分析表明，苯丙烷类生物合成和植物激素信号转导显著上调。ABA依赖途径对于抗旱和酚酸积累至关重要。干旱和ABA数据库中的表达模式表明，四个PYLs对干旱和ABA均有响应，并对三个潜在的SnRK2家族成员进行了注释和分析。本研究对受干旱影响的[具体植物名称未给出]进行了全面的转录组分析，为理解[具体植物名称未给出]面对非生物胁迫的分子机制提供了丰富资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/786be0e5f9c6/plants-13-00161-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/5042df7e6410/plants-13-00161-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/ddcadfa09d2a/plants-13-00161-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/367bf02ec574/plants-13-00161-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/a99809fa4b75/plants-13-00161-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/e10050941288/plants-13-00161-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/636d224c7c92/plants-13-00161-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/1bcde6dc279e/plants-13-00161-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/786be0e5f9c6/plants-13-00161-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/5042df7e6410/plants-13-00161-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/ddcadfa09d2a/plants-13-00161-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/367bf02ec574/plants-13-00161-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/a99809fa4b75/plants-13-00161-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/e10050941288/plants-13-00161-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/636d224c7c92/plants-13-00161-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/1bcde6dc279e/plants-13-00161-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b209/10819027/786be0e5f9c6/plants-13-00161-g008.jpg

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