Darczuk D, Krzysciak W, Vyhouskaya P, Kesek B, Galecka-Wanatowicz D, Lipska W, Kaczmarzyk T, Gluch-Lutwin M, Mordyl B, Chomyszyn-Gajewska M
Department of Periodontology and Oral Medicine, Jagiellonian University Medical College, Cracow, Poland.
Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Medical College, Cracow, Poland.
J Physiol Pharmacol. 2016 Dec;67(6):885-894.
Reactive oxygen species (ROS) are involved in the pathogenesis of many inflammatory diseases, including oral lichen planus. Therefore, determining the salivary markers of oxidative stress is an excellent alternative approach to diagnosing oral cavity diseases. The objective of our study was to provide preliminary validation and determination of the salivary markers of oxidative stress in both patients with reticular and erosive forms of oral lichen planus as well as in healthy individuals without any oral lesions. In total, 62 patients with oral lichen planus (OLP) were enrolled in the study, including 31 with the reticular form of lichen planus (44.63 ± 11.05 years) and 31 with erosive forms (40.43 ± 10.05 years), who had never been treated for their disease. The control group comprised 30 individuals without any oral lesions (42.12 ± 12.22 years). We determined the saliva levels in glutathione (GSH), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS). The mean saliva levels of GSH and TAC were significantly lower (P < 0.01) in OLP patients compared to the control group. The mean levels of salivary TBARS were higher in both OLP groups (reticular and erosive) compared to the control group (P = 0.01). The lower saliva levels of GSH and TAC in patients with OLP indicate that free radicals and the resulting oxidative damage may play an important role in the pathogenesis of OLP lesions. In conclusion, monitoring the oxidant-antioxidant status of saliva may serve as an efficient and less intrusive marker for determining stages of disease development in patients with OLP.
活性氧（ROS）参与包括口腔扁平苔藓在内的多种炎症性疾病的发病机制。因此，确定氧化应激的唾液标志物是诊断口腔疾病的一种极佳替代方法。我们研究的目的是对口腔扁平苔藓网状型和糜烂型患者以及无任何口腔病变的健康个体的氧化应激唾液标志物进行初步验证和测定。本研究共纳入62例口腔扁平苔藓（OLP）患者，其中31例为扁平苔藓网状型（44.63±11.05岁），31例为糜烂型（40.43±10.05岁），这些患者从未接受过该病的治疗。对照组包括30例无任何口腔病变的个体（42.12±12.22岁）。我们测定了谷胱甘肽（GSH）、总抗氧化能力（TAC）和硫代巴比妥酸反应性物质（TBARS）的唾液水平。与对照组相比，OLP患者的GSH和TAC平均唾液水平显著降低（P<0.01）。与对照组相比，两个OLP组（网状型和糜烂型）的唾液TBARS平均水平均较高（P = 0.01）。OLP患者唾液中GSH和TAC水平较低表明自由基及由此产生的氧化损伤可能在OLP病变的发病机制中起重要作用。总之，监测唾液的氧化-抗氧化状态可能是确定OLP患者疾病发展阶段的一种有效且侵入性较小的标志物。
