Malmhäll C, Johansson K, Winkler C, Alawieh S, Ekerljung L, Rådinger M
Krefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Astra Zeneca, Mölndal, Sweden.
Scand J Immunol. 2017 Apr;85(4):300-307. doi: 10.1111/sji.12535.
We and others have previously identified microRNAs (miRNAs) with pathological roles in animal models of asthma, where miR-146a and miR-155 have been described to play important roles in inflammatory responses. To date, few studies have investigated miRNA expression in human asthmatics. In the current study, significantly lower levels of miR-155 were detected in cell-free sputum from allergic asthmatics compared to healthy controls. Induced sputum isolated from allergic asthmatics in and out of pollen season revealed that miR-155 expression, but not miR-146a, is reduced in lymphocytes in season compared to post-season. In contrast, miR-155 was found to increase, whereas miR-146a decreased in PBMCs and cell-free PBMC culture media upon T cell receptor stimulation via αCD3/CD28 in both allergic asthmatics and healthy controls. Our findings suggest that miR-155 is differentially expressed ex vivo in airways of allergic asthmatics compared to healthy controls, which may have implications in the local immune response in allergic asthma.
我们和其他研究人员之前已在哮喘动物模型中鉴定出具有病理作用的微小RNA（miRNA），其中miR-146a和miR-155已被描述在炎症反应中发挥重要作用。迄今为止，很少有研究调查人类哮喘患者中miRNA的表达情况。在本研究中，与健康对照相比，在过敏性哮喘患者的无细胞痰液中检测到显著更低水平的miR-155。从花粉季节期间及之后的过敏性哮喘患者中分离出的诱导痰液显示，与花粉季节之后相比，花粉季节期间淋巴细胞中的miR-155表达降低，但miR-146a表达未降低。相反，在过敏性哮喘患者和健康对照中，经αCD3/CD28刺激T细胞受体后，外周血单核细胞（PBMC）及无细胞PBMC培养基中的miR-155增加，而miR-146a减少。我们的研究结果表明，与健康对照相比，过敏性哮喘患者气道中miR-155在体外存在差异表达，这可能对过敏性哮喘的局部免疫反应有影响。
