Boonruamkaew Phetcharat, Sukketsiri Wanida, Panichayupakaranant Pharkphoom, Kaewnam Wijittra, Tanasawet Supita, Tipmanee Varomyalin, Hutamekalin Pilaiwanwadee, Chonpathompikunlert Pennapa
Department of Physiology, Faculty of Science, Prince of Songkla University, Hat Yai, 90110, Songkhla, Thailand.
Department of Pharmacology, Faculty of Science, Prince of Songkla University, Hat Yai, 90110, Songkhla, Thailand.
J Nat Med. 2017 Jul;71(3):492-505. doi: 10.1007/s11418-017-1077-6. Epub 2017 Feb 15.
Apium graveolens is a food flavoring which possesses various health promoting effects. This study investigates the effect of a sub-acute administration of A. graveolens on cognition and anti-depression behaviors via antioxidant and related neurotransmitter systems in mice brains. Cognition and depression was assessed by various models of behavior. The antioxidant system of glutathione peroxidase (GPx), % inhibition of superoxide anion (O), and lipid peroxidation were studied. In addition, neurochemical parameters including acetylcholinesterase (AChE) and monoamine oxidase-type A (MAO-A) were also evaluated. Nine groups of male mice were fed for 30 days with different substances-a control, vehicle, A. graveolens extract (65-500 mg/kg), and reference drugs (donepezil and fluoxetine). The results indicated that the effect of the intake of A. graveolens extract (125-500 mg/kg) was similar to the reference drugs, as it improved both spatial and non-spatial memories. Moreover, there was a decrease in immobility time in both the forced swimming and tail suspension tests. In addition, the A. graveolens extract reduced lipid peroxidation of the brain and increased GPx activity and the % inhibition of O, whereas the activities of AChE and MAO-A were decreased. Thus, our data have shown that the consumption of A. graveolens extract improved cognitive function and anti-depression activities as well as modulating the endogenous antioxidant and neurotransmitter systems in the brain, resulting in increased neuronal density. This result indicated an important role for A. graveolens extract in preventing age-associated decline in cognitive function associated with depression.
芹菜是一种具有多种促进健康作用的食品调味剂。本研究通过小鼠大脑中的抗氧化剂及相关神经递质系统，探讨亚急性给予芹菜对认知和抗抑郁行为的影响。通过多种行为模型评估认知和抑郁情况。研究了谷胱甘肽过氧化物酶（GPx）的抗氧化系统、超氧阴离子（O）的抑制率以及脂质过氧化情况。此外，还评估了包括乙酰胆碱酯酶（AChE）和单胺氧化酶A 型（MAO -A）在内的神经化学参数。将九组雄性小鼠分别用不同物质喂养30天，包括对照组、赋形剂组、芹菜提取物（65 - 500毫克/千克）以及参考药物（多奈哌齐和氟西汀）。结果表明，摄入芹菜提取物（125 - 500毫克/千克）的效果与参考药物相似，因为它改善了空间和非空间记忆。此外，在强迫游泳和悬尾试验中，不动时间均有所减少。此外，芹菜提取物降低了大脑的脂质过氧化，增加了GPx活性和O的抑制率，而AChE和MAO -A的活性则降低。因此，我们的数据表明，食用芹菜提取物可改善认知功能和抗抑郁活性，并调节大脑中的内源性抗氧化剂和神经递质系统，从而增加神经元密度。这一结果表明芹菜提取物在预防与抑郁相关的认知功能随年龄下降方面具有重要作用。
