ICAR- National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.
Indian Institute of Wheat and Barley Research, Regional Station Flowerdale, Shimla, H.P., India.
Sci Rep. 2017 Feb 17;7:42419. doi: 10.1038/srep42419.
Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici, is one of the important diseases of wheat. We used NGS technologies to generate a draft genome sequence of two highly virulent (46S 119 and 31) and a least virulent (K) pathotypes of P. striiformis from the Indian subcontinent. We generated ~24,000-32,000 sequence contigs (N50;7.4-9.2 kb), which accounted for ~86X-105X sequence depth coverage with an estimated genome size of these pathotypes ranging from 66.2-70.2 Mb. A genome-wide analysis revealed that pathotype 46S 119 might be highly evolved among the three pathotypes in terms of year of detection and prevalence. SNP analysis revealed that ~47% of the gene sets are affected by nonsynonymous mutations. The extracellular secreted (ES) proteins presumably are well conserved among the three pathotypes, and perhaps purifying selection has an important role in differentiating pathotype 46S 119 from pathotypes K and 31. In the present study, we decoded the genomes of three pathotypes, with 81% of the total annotated genes being successfully assigned functional roles. Besides the identification of secretory genes, genes essential for pathogen-host interactions shall prove this study as a huge genomic resource for the management of this disease using host resistance.
小麦条锈病由条形柄锈菌小麦专化型引起，是小麦的重要病害之一。我们使用 NGS 技术，从印度次大陆生成了两个高毒性（46S119 和 31）和一个低毒性（K）生理小种的条形柄锈菌草案基因组序列。我们生成了约 24000-32000 个序列片段（N50；7.4-9.2 kb），序列深度覆盖率约为 86X-105X，估计这些生理小种的基因组大小在 66.2-70.2 Mb 之间。全基因组分析表明，在三个生理小种中，46S119 可能是进化程度最高的一个，这与检测年份和流行程度有关。SNP 分析表明，约 47%的基因集受到非同义突变的影响。推测三个生理小种之间的细胞外分泌（ES）蛋白是高度保守的，也许纯化选择在将 46S119 生理小种与 K 和 31 生理小种区分开来方面起着重要作用。在本研究中，我们对三个生理小种的基因组进行了解码，总注释基因中有 81%被成功赋予了功能作用。除了鉴定分泌基因外，对病原体-宿主相互作用至关重要的基因将证明这项研究是一个巨大的基因组资源，可用于利用宿主抗性来管理这种疾病。
