重组痘苗病毒表达传染性支气管炎病毒刺突蛋白及在接种小鼠中诱导中和抗体

Expression of the infectious bronchitis virus spike protein by recombinant vaccinia virus and induction of neutralizing antibodies in vaccinated mice.

作者信息

Tomley F M, Mockett A P, Boursnell M E, Binns M M, Cook J K, Brown T D, Smith G L

机构信息

Houghton Poultry Research Station, Huntingdom, Cambridgeshire, U.K.

出版信息

J Gen Virol. 1987 Sep;68 ( Pt 9):2291-8. doi: 10.1099/0022-1317-68-9-2291.

DOI:10.1099/0022-1317-68-9-2291

PMID:2821170

Abstract

A cDNA clone of the infectious bronchitis virus (IBV) spike protein gene has been recombined into vaccinia virus. Cells infected with the recombinant virus synthesized IBV spike antigen which was recognized by antibody raised against purified spike protein. Immunofluorescence showed that the IBV spike antigen was transported to the infected cell surface membrane and immunoprecipitation showed the presence of the glycosylated 180K mol. wt. polypeptide precursor of the two spike subunits S1 and S2 that comigrated with this antigen from IBV-infected cells. Vaccinated mice produced antibody that recognized the IBV spike antigen by ELISA and which neutralized IBV infectivity as shown by ciliostasis tests on tracheal organ cultures.

摘要

传染性支气管炎病毒（IBV）刺突蛋白基因的一个cDNA克隆已被重组到痘苗病毒中。用重组病毒感染的细胞合成了IBV刺突抗原，该抗原可被针对纯化刺突蛋白产生的抗体识别。免疫荧光显示，IBV刺突抗原被转运到受感染细胞的表面膜，免疫沉淀显示存在糖基化的180K分子量的两种刺突亚基S1和S2的多肽前体，其与来自IBV感染细胞的该抗原共迁移。接种疫苗的小鼠产生的抗体通过ELISA识别IBV刺突抗原，并如气管器官培养物上的纤毛停滞试验所示中和IBV感染性。