Department of Prosthetic Dentistry, Regensburg University Medical Center, Regensburg, Germany.
Department of Biomedical, Surgical and Dental Sciences, IRCCS Galeazzi Institute, University of Milan, Italy.
J Dent. 2017 May;60:14-24. doi: 10.1016/j.jdent.2017.02.005. Epub 2017 Feb 14.
To elucidate the impact of surface properties and the release of fluoride from different glass ionomer cements on biofilm formation.
Standardized specimens manufactured from various classes of glass ionomer cements (GICs), a resin-based composite (RBC), and human enamel were subjected to surface analyses. Subsequent to simulation of salivary pellicle formation, Streptococcus mutans biofilm formation was initiated using a drip flow reactor for 48h and 96h. Biofilms were characterized by determining viable bacterial biomass and 3D biofilm architecture using SEM and CLSM; the release of fluoride from the specimens was measured using the ion selective micro method in dependence on various experimental conditions (incubation with sterile broth/bacteria/acid).
Surface properties and biofilm formation correlated poorly, while the release of fluoride correlated well with viable streptococcal biomass and SEM/CLSM analyses. For all investigated materials, biofilm formation was lower than on enamel. The release of fluoride showed a significant dependency on the experimental conditions applied; the presence of biofilms reduced fluoride release in comparison to sterile incubation conditions.
Within the limitations of a laboratory study, the results suggest that biofilm formation on GICs cannot be easily predicted as a function of substratum surface parameters. The release of fluoride from glass ionomer cements contributes to control biofilm formation particularly in its early phases.
Glass ionomer cements can actively control microbial biofilm formation, while biofilms modulate the release of fluoride from GIC materials.
阐明不同玻璃离子水门汀的表面性质和氟释放对生物膜形成的影响。
用各种类型的玻璃离子水门汀（GIC）、树脂基复合材料（RBC）和人牙釉质制成标准试件，进行表面分析。在模拟唾液膜形成后，使用滴流反应器启动变形链球菌生物膜形成，持续 48 小时和 96 小时。使用 SEM 和 CLSM 测定活细菌生物量和 3D 生物膜结构来表征生物膜；使用离子选择性微方法测量标本中氟的释放，根据不同的实验条件（无菌肉汤/细菌/酸孵育）进行测量。
表面性质和生物膜形成相关性较差，而氟释放与链球菌活生物量和 SEM/CLSM 分析相关性较好。对于所有研究材料，生物膜形成均低于牙釉质。氟的释放显示出对所应用实验条件的显著依赖性；与无菌孵育条件相比，生物膜的存在会降低氟的释放。
在实验室研究的限制范围内，结果表明，GIC 上的生物膜形成不能简单地作为基底表面参数的函数来预测。玻璃离子水门汀的氟释放有助于控制生物膜形成，特别是在其早期阶段。
玻璃离子水门汀可以主动控制微生物生物膜的形成，而生物膜会调节 GIC 材料中氟的释放。
