óDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, 627 412 Tirunelveli, Tamil Nadu, India.
óDivision of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, 627 412 Tirunelveli, Tamil Nadu, India.
Ecotoxicol Environ Saf. 2017 May;139:439-446. doi: 10.1016/j.ecoenv.2017.01.026. Epub 2017 Feb 23.
Resistance to treatments with Temephos or plant derived oil, Pb-CVO, between a field collected Wild Strain (WS) and a susceptible Laboratory Strain (LS) of Ae. aegypti were measured. The Temephos (0.1mg/L) showed the greatest percentage of mosquito mortality compared to Pb-CVO (1.5mg/L) in LS Ae. aegypti. However, WS Ae. aegypti was not significantly affected by Temephos (0.1mg/L) treatment compare to the Pb-CVO (1.5mg/L). However, both strains (LS and WS) when treated with Pb-CVO (1.5mg/L) displayed steady larval mortality rate across all instars. The LC of Temephos was 0.027mg in LS, but increased in WS to 0.081mg/L. The LC of Pb-CVO treatment was observed at concentrations of 0.72 and 0.64mg/L for LS and WS strains respectively. The enzyme level of α- and β-carboxylesterase was reduced significantly in both mosquito strains treated with Pb-CVO. Whereas, there was a prominent deviation in the enzyme ratio observed between LS and WS treated with Temephos. The GST and CYP450 levels were upregulated in the LS, but decreased in WS, after treatment with Temephos. However, treatment with Pb-CVO caused both enzyme levels to increase significantly in both the strains. Visual observations of the midgut revealed cytotoxicity from sub-lethal concentrations of Temephos (0.04mg/L) and Pb-CVO (1.0mg/L) in both strains of Ae. aegypti compared to the control. The damage caused by Temephos was slightly less in WS compared to LS mosquito strains.
测定了野外采集的野生株（WS）和敏感实验室株（LS）埃及伊蚊对涕灭威或植物源油 Pb-CVO 治疗的抗性。与 LS 埃及伊蚊中的 Pb-CVO（1.5mg/L）相比，涕灭威（0.1mg/L）对蚊子的死亡率最高。然而，与 Pb-CVO（1.5mg/L）处理相比，WS 埃及伊蚊对涕灭威（0.1mg/L）处理的影响并不显著。然而，当用 Pb-CVO（1.5mg/L）处理时，两种菌株（LS 和 WS）均表现出整个龄期稳定的幼虫死亡率。LS 中的涕灭威 LC 为 0.027mg，但在 WS 中增加到 0.081mg/L。LC 观察到 LS 和 WS 菌株的 Pb-CVO 处理浓度分别为 0.72 和 0.64mg/L。用 Pb-CVO 处理后，两种蚊子的α-和β-羧酸酯酶的酶水平显著降低。然而，在用涕灭威处理的 LS 和 WS 之间观察到酶比的明显偏差。在用涕灭威处理后，LS 的 GST 和 CYP450 水平上调，但 WS 下降。然而，Pb-CVO 处理导致两种菌株的酶水平均显著增加。在用亚致死浓度的涕灭威（0.04mg/L）和 Pb-CVO（1.0mg/L）处理后，观察到 LS 和 WS 埃及伊蚊的中肠出现细胞毒性，与对照相比。与 LS 蚊子株相比，WS 蚊子株中涕灭威引起的损伤略小。
