Dos Santos Larissa Sarmento, Sá Joicy Cortez, Dos Santos Ribeiro Diego Luiz, Chaves Nancyleni Pinto, da Silva Mol Juliana Pinto, Santos Renato Lima, da Paixão Tatiane Alves, de Carvalho Neta Alcina Vieira
Doutorado em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Estadual do Maranhão, São Luís, MA, Brazil.
Departamento do Curso de Medicina, Universidade Ceuma, São Luís, MA, Brazil.
Trop Anim Health Prod. 2017 Apr;49(4):675-679. doi: 10.1007/s11250-017-1238-3. Epub 2017 Feb 18.
The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.
本研究的目的是使用血清学、细菌分离和分子分析等方法，对马拉尼昂州养殖的水牛进行布鲁氏菌属感染诊断。为此，对390份水牛血清样本进行了血清学检测、玫瑰红平板试验（RBPT）以及2-巯基乙醇（2-ME）联合迟缓凝集试验（SAT）。从血清学阳性动物采集阴道拭子，进行细菌分离和通用PCR。根据血清学检测，16只动物的确诊试验（2-ME/SAT）呈阳性反应。在细菌分离方面，三份样本分离出了具有布鲁氏菌属特征的菌落，并通过PCR得到确认。这些结果证实了马拉尼昂州水牛群中存在布鲁氏菌属感染。
