Kita J, Balasuriya U B, Osburn B I
Department of Infectious Diseases, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.
Arch Vet Pol. 1994;34(3-4):195-204.
Bovine and ovine parainfluenza-3 (PI-3) virus proteins were compared using polyclonal and monoclonal antibodies in western blot analysis. Eight proteins ranging in molecular weight (MW) from 14 kD to 190 kD of bovine PI-3 virus were demonstrated in this study. The 14 kD MW protein has not been previously described for bovine PI-3 virus. Western blot analysis of ovine PI-3 virus using homologous serum produced 7 bands ranging in MW from 12 kD to 190 kD described for the first time in this paper. Using rabbit polyclonal antibodies against bovine PI-3 virus a 160 kD MW protein was demonstrated in the bovine virus strain, but not in the ovine PI-3 virus strain and a protein band appeared in the ovine PI-3 virus strain at a MW of 23 kD but not in the bovine PI-3 virus strain. Using lamb polyclonal antibodies to ovine PI-3 virus against the homologous virus, 6 protein bands were visible with a MW range from 12 kD to 175 kD; for bovine PI-3 virus 5 protein bands were resolved. There was a difference observed in the mobility of the 120 kD MW protein for ovine PI-3 viral protein but not for the bovine PI-3 viral protein. Differences were observed for ovine PI-3 viral protein at the level of the 12 kD MW protein whereas the lowest detected band for the bovine PI-3 viral protein was 37 kD MW. The monoclonal antibodies prepared against bovine PI-3 virus produced 3 distinct protein bands. Two bands of MW 110 and 140 kD were identical for both viruses whereas the third band for bovine PI-3 virus was 37 kD MW and that for ovine PI-3 virus was 55 kD MW.
在蛋白质印迹分析中，使用多克隆抗体和单克隆抗体对牛和羊的副流感3型（PI-3）病毒蛋白进行了比较。本研究证实了牛PI-3病毒的8种分子量（MW）在14 kD至190 kD之间的蛋白质。14 kD MW的蛋白质此前未在牛PI-3病毒中被描述过。使用同源血清对羊PI-3病毒进行蛋白质印迹分析，产生了7条分子量在12 kD至190 kD之间的条带，本文首次对此进行了描述。使用抗牛PI-3病毒的兔多克隆抗体，在牛病毒株中证实了一种160 kD MW的蛋白质，但在羊PI-3病毒株中未检测到；在羊PI-3病毒株中出现了一条23 kD MW的蛋白带，而在牛PI-3病毒株中未出现。使用抗羊PI-3病毒的羊多克隆抗体检测同源病毒时，可见6条分子量在12 kD至175 kD之间的蛋白带；对于牛PI-3病毒，分辨出了5条蛋白带。观察到羊PI-3病毒的120 kD MW蛋白的迁移率存在差异，而牛PI-3病毒的该蛋白迁移率无差异。在12 kD MW蛋白水平上观察到羊PI-3病毒蛋白存在差异，而牛PI-3病毒蛋白检测到的最低条带为37 kD MW。针对牛PI-3病毒制备的单克隆抗体产生了3条不同的蛋白带。两种病毒中分子量为110和140 kD的两条带相同，而牛PI-3病毒的第三条带为37 kD MW，羊PI-3病毒的第三条带为55 kD MW。
