Stratton C W, Liu C, Ratner H B, Weeks L S
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
Antimicrob Agents Chemother. 1987 Jul;31(7):1014-6. doi: 10.1128/AAC.31.7.1014.
This study used kill-kinetic methods to provide data on the bactericidal activity of subinhibitory (1/2 X MIC), inhibitory (1 x MIC), and suprainhibitory (4X, 6X, and 8X MIC) concentrations of deptomycin (LY146032) against strains of enterococci compared with those of ciprofloxacin, vancomycin, and ampicillin. Deptomycin was the most active agent tested, as determined by broth microdilution methods, with all strains being inhibited at concentrations less than or equal to 2 micrograms/ml. The kill-kinetic demonstrated that deptomycin had greater activity at all concentrations tested than the other cell wall-active agents; regrowth was seen, however, at lower concentrations. At higher concentrations (6X and 8X MIC), all agents tested demonstrated the same or less bactericidal activity than at 4X MIC, presumably due to the Eagle effect. Nevertheless, these results suggest that further evaluation of deptomycin as a therapeutic agent for serious enterococcal infections is warranted.
本研究采用杀菌动力学方法，提供了去甲万古霉素（LY146032）的亚抑菌浓度（1/2×最低抑菌浓度）、抑菌浓度（1×最低抑菌浓度）以及超抑菌浓度（4×、6×和8×最低抑菌浓度）对肠球菌菌株的杀菌活性数据，并与环丙沙星、万古霉素和氨苄西林进行了比较。通过肉汤微量稀释法测定，去甲万古霉素是所测试的最具活性的药物，所有菌株在浓度小于或等于2微克/毫升时均受到抑制。杀菌动力学表明，去甲万古霉素在所有测试浓度下的活性均高于其他细胞壁活性药物；然而，在较低浓度下可见细菌再生长。在较高浓度（6×和8×最低抑菌浓度）时，所有测试药物的杀菌活性与4×最低抑菌浓度时相同或更低，这可能是由于伊格尔效应。尽管如此，这些结果表明去甲万古霉素作为严重肠球菌感染治疗药物值得进一步评估。
