Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
Nat Chem Biol. 2017 Apr;13(4):360-362. doi: 10.1038/nchembio.2301. Epub 2017 Feb 20.
Fungal type I fatty acid synthases (FASs) are mega-enzymes with two separated, identical compartments, in which the acyl carrier protein (ACP) domains shuttle substrates to catalytically active sites embedded in the chamber wall. We devised synthetic FASs by integrating heterologous enzymes into the reaction chambers and demonstrated their capability to convert acyl-ACP or acyl-CoA from canonical fatty acid biosynthesis to short/medium-chain fatty acids and methyl ketones.
真菌 I 型脂肪酸合酶（FASs）是具有两个分离且相同隔室的巨型酶，其中酰基载体蛋白（ACP）结构域将底物穿梭到嵌入腔壁的催化活性位点。我们通过将异源酶整合到反应室中设计了合成 FASs，并证明了它们将酰基-ACP 或酰基辅酶 A 从典型脂肪酸生物合成转化为短/中链脂肪酸和甲基酮的能力。
