Department of Forensic Medicine, College of Basic Medicine, Chongqing Medical University, Chongqing, 400016, China.
Department of Forensic Genetics, Institute of Forensic Science, Guangzhou Public Security Bureau, Guangzhou, Guangdong, 510000, China.
Int J Legal Med. 2017 Nov;131(6):1539-1542. doi: 10.1007/s00414-017-1577-7. Epub 2017 Mar 24.
The allele frequencies and forensic statistical parameters of 19 autosomal short tandem repeat (STR) loci (D8S1179, D21S11, D7S820, CSF1P0, D3S1358, THOl, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, D6S1043, Penta D, Penta E, and D12S391) included in the Goldeneye™ DNA ID system 20A kit were obtained in 671 Chinese Han individuals residing in Chongqing, Southwest China. All 19 STR loci were identified in agreement with the Hardy-Weinberg equilibrium. A total of 238 alleles were observed with corresponding allele frequencies that varied from 0.0007 to 0.5119. The combined power of discrimination and the combined probability of exclusion for 19 STR loci in the Chongqing Han population were 0.99999999999999999999998954 and 0.99999998387, respectively. The findings indicated that the 19 autosomal STR loci were highly polymorphic in the Chongqing Han population and can be used as a powerful tool in personal identification and parentage testing. Our genetic study enriched the Chinese local forensic reference database. Population comparisons and phylogenetic analyses revealed that genetic heterogeneity widely existed among the Chongqing Han, Xinjiang Uyghur, and Kazakh populations as well as demonstrated that genetic similarity was tightly associated with those of close geographic origin or of the same ethnic origin.
在中国西南部重庆市，对 671 名汉族个体的金睛 DNA ID 系统 20A 试剂盒中的 19 个常染色体短串联重复序列（STR）基因座（D8S1179、D21S11、D7S820、CSF1P0、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA、D6S1043、Penta D、Penta E 和 D12S391）的等位基因频率和法医统计学参数进行了研究。所有 19 个 STR 基因座均符合 Hardy-Weinberg 平衡。共观察到 238 个等位基因，对应的等位基因频率从 0.0007 到 0.5119 不等。19 个 STR 基因座在重庆汉族人群中的鉴别力总和和排除概率总和分别为 0.9999999999999999999998954 和 0.99999998387。研究结果表明，19 个常染色体 STR 基因座在重庆汉族人群中具有高度多态性，可作为个人识别和亲子鉴定的有力工具。本遗传研究丰富了中国本地法医参考数据库。群体比较和系统进化分析表明，重庆汉族、新疆维吾尔族和哈萨克族之间存在广泛的遗传异质性，并且遗传相似性与地理起源或民族起源密切相关。
