[人骨髓间充质干细胞成脂分化过程中miR-140-5p的表达及其靶基因预测]
[Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation].
作者信息
Wang Tao, Yan Rui-Qiao, Cao Jun, Cao Ling-Ling, Zhang Xuan-Pu, Li Xing-Nuan, Wu Ping, Zhou Xiao-Ou, Wu Jian-Fang, Xu Xiao-Yuan
机构信息
1Key Laboratory of Jiangxi Province for Systems Biomedicine, Jiujiang 332000, China; 2Department of Pathogenic Biology, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2016 Feb 20;37(2):199-203. doi: 10.3969/j.issn.1673-4254.2017.02.09.
OBJECTIVE
To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation.
METHODS
Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest. The databases including TargetScan, PicTar and miRanda were used to predict the target genes of the differentially expressed miRNA.
RESULTS
The expression of miR-140-5p was down-regulated and leukemia inhibitory factor receptor (LIFR) expression increased progressively during adipogenic differentiation of hMSCs, showing a negative correlation between them. Target gene prediction using the 3 databases identified LIFR as the target gene of miR-140-5p.
CONCLUSION
miRNA-140-5p may play an important role by regulating its target gene LIFR during adipogenic differentiation of hMSCs.
目的
筛选人骨髓间充质干细胞（hMSCs）成脂分化过程中差异表达的微小RNA（miRNAs）及其靶基因，以更好地理解调节成骨细胞与脂肪细胞分化平衡的机制。
方法
培养hMSCs并诱导其成脂分化，在诱导的第0、7、14和21天，使用miRNA芯片和转录组测序（RNA-seq）技术检测细胞的miRNA和mRNA表达谱。对潜在感兴趣的miRNA和mRNA进行相关性分析。利用TargetScan、PicTar和miRanda等数据库预测差异表达miRNA的靶基因。
结果
在hMSCs成脂分化过程中，miR-140-5p表达下调，白血病抑制因子受体（LIFR）表达逐渐增加，二者呈负相关。使用这3个数据库进行靶基因预测，确定LIFR为miR-140-5p的靶基因。
结论
miRNA-140-5p可能通过在hMSCs成脂分化过程中调节其靶基因LIFR发挥重要作用。
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