Borges Karen Apellanis, Furian Thales Quedi, de Souza Sara Neves, Tondo Eduardo César, Streck André Felipe, Salle Carlos Tadeu Pippi, de Souza Moraes Hamilton Luiz, do Nascimento Vladimir Pinheiro
Centro de Diagnóstico e Pesquisa em Patologia Aviária (CDPA), Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gonçalves 8824, Porto Alegre, Rio Grande do Sul 91540-000, Brazil.
Instituto de Ciência e Tecnologia dos Alimentos (ICTA), Universidade Federal do Rio Grande do Sul (UFRGS), Agronomia, Avenida Bento Gonçalves 9500, Porto Alegre, Rio Grande do Sul 91501-970, Brazil.
J Food Prot. 2017 Jan;80(1):158-163. doi: 10.4315/0362-028X.JFP-16-299.
Salmonella spp. are among the most important agents of foodborne diseases all over the world. Human Salmonella outbreaks are often associated with the consumption of poultry products (meat and eggs), and one of the most prevalent serotypes associated with these products is Salmonella Enteritidis. Brazil is one of the most important poultry exporters in the world. In southern Brazil, three closely related clones of Salmonella Enteritidis have been responsible for the majority of foodborne Salmonella outbreaks over the past decade. However, until now, there has been little information regarding the clonal relationship among the Brazilian Salmonella strains of avian origin and those involved in foodborne outbreaks. Therefore, the aim of the present study was to complete the molecular characterization of Salmonella Enteritidis strains isolated from poultry and food sources involved in Salmonella outbreaks. PCR ribotyping was performed to discriminate the strains into different ribotype profiles according to the banding pattern amplification. This technique was able to differentiate the Salmonella Enteritidis strains into two banding patterns: R2 and R4. R2 accounted for 98.7% of the strains. DNA sequencing of the 600-bp fragment, present in all ribotypes, was applied to confirm this result. The sequences generated showed high levels of similarity, ranging from 99.7 to 100%, and were grouped into a single cluster. These results suggest that there is a clonal relationship among the Salmonella Enteritidis strains responsible for several salmonellosis outbreaks and the strains collected from poultry sources.
沙门氏菌属是全球食源性疾病最重要的病原体之一。人类沙门氏菌疫情通常与食用禽肉产品（肉和蛋）有关，与这些产品相关的最常见血清型之一是肠炎沙门氏菌。巴西是世界上最重要的禽肉出口国之一。在巴西南部，三种密切相关的肠炎沙门氏菌克隆在过去十年中导致了大多数食源性沙门氏菌疫情。然而，到目前为止，关于巴西禽源沙门氏菌菌株与食源性疫情中涉及的菌株之间的克隆关系，几乎没有相关信息。因此，本研究的目的是完成从参与沙门氏菌疫情的家禽和食物来源中分离出的肠炎沙门氏菌菌株的分子特征分析。根据条带模式扩增，采用PCR核糖体分型法将菌株分为不同的核糖体分型谱。该技术能够将肠炎沙门氏菌菌株分为两种条带模式：R2和R4。R2占菌株的98.7%。对所有核糖体分型中存在的600 bp片段进行DNA测序以证实这一结果。生成的序列显示出高度相似性，范围从99.7%到100%，并被归为一个单一的簇。这些结果表明，导致几次沙门氏菌病疫情的肠炎沙门氏菌菌株与从家禽来源收集的菌株之间存在克隆关系。
