Ge Ye, Chai Hongliang, Fan Zhiqiang, Wang Xianfu, Yao Qiucheng, Ma Jian, Chen Si, Hua Yuping, Deng Guohua, Chen Hualan
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China.
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang Province, China.
Virol J. 2017 Feb 21;14(1):36. doi: 10.1186/s12985-017-0680-1.
H6 subtype avian influenza viruses are globally distributed and, in recent years, have been isolated with increasing frequency from both domestic and wild bird species as well as infected humans. Many reports have examined the viruses in the context of poultry or several wild bird species, but there is less information regarding their presence in migratory birds.
Hemagglutination and hemagglutination inhibition tests were used to measure HA activity for different HA subtypes. Whole viral genomes were sequenced and analysed using DNAstar and MEGA 6 to understand their genetic evolution. Pathogenicity was evaluated using a mouse infection model.
We isolated 13 strains of H6 virus from faecal samples of migratory waterfowl in Anhui Province of China in 2014. Phylogenetic analysis showed gene reassortment between Eurasian and North American lineages. Five of the identified H6 strains had the ability to infect mice without adaptation.
Our findings suggest that regular surveillance of wild birds, especially migratory birds, is important for providing early warning and control of avian influenza outbreaks.
H6亚型禽流感病毒在全球范围内均有分布，近年来，在家禽和野鸟以及感染人类中分离出该病毒的频率不断增加。许多报告已在禽类或几种野鸟的背景下对这些病毒进行了研究，但关于它们在候鸟中的存在情况的信息较少。
采用血凝和血凝抑制试验来测量不同HA亚型的HA活性。使用DNAstar和MEGA 6对完整病毒基因组进行测序和分析，以了解其遗传进化。使用小鼠感染模型评估致病性。
2014年，我们从中国安徽省候鸟的粪便样本中分离出13株H6病毒。系统发育分析显示欧亚和北美谱系之间存在基因重配。所鉴定的13株H6病毒中有5株能够直接感染小鼠。
我们的研究结果表明，对野生鸟类，尤其是候鸟进行定期监测对于提供禽流感爆发的早期预警和控制非常重要。
