Al-Massarani Shaza M, El Gamal Ali A, Abd El Halim Mohamed F, Al-Said Mansour S, Abdel-Kader Maged S, Basudan Omer A, Alqasoumi Saleh I
Pharmacognosy Department, Faculty of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Pharmacognosy Department, Faculty of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, P.O. 35516, Mansoura, Egypt.
Saudi Pharm J. 2017 Jan;25(1):110-119. doi: 10.1016/j.jsps.2016.05.006. Epub 2016 May 24.
The total extract of flowers was examined for its possible hepatoprotective activity in comparison with the standard drug silymarin at two doses. The higher dose expressed promising activity especially in reducing the levels of AST, ALT and bilirubin. Fractionation via liquid-liquid partition and reexamination of the fractions revealed that the -butanol fraction was the best in improving liver biochemical parameters followed by the -hexane fraction. However, serum lipid parameters were best improved with CHCl fraction. The promising biological activity results initiated an intensive chromatographic purification of flowers fractions. Two compounds were identified from natural source for the first time, the acyclic farnesyl sesquiterpene glycoside1-O-[6-O--l-arabinopyranosyl--d-glucopyranoside]-(2,6-)-farnesol () and the squalene derivative 2,3-dihydroxy-2,3-dihydrosqualene (), in addition to eight compounds reported here for the first time from the genus ; two benzyl glycosides, benzyl 1-O--d-glucopyranoside () and benzyl 6-O--l-arabinopyranosyl -d-glucopyranoside (); three acyclic monoterpene glycosides, linalyl -d-glucopyranoside () and linalyl 6-O--l-arabinopyranosyl--d-glucopyranoside (); (2)-3,7-dimethylocta-2,6-dienoate-6-O--l arabinopyranosyl--d-glucopyranoside (), two oligoglycosides, -hexyl--l arabinopyranosyl-(1 → 6)--d-glucopyranoside (creoside) () and -octyl -l-arabinopyranosyl-(1 → 6)--d-glucopyranoside (rhodiooctanoside) (); and ethyl fructofuranoside (). The structures of the isolated compounds were elucidated based on extensive examination of their spectroscopic 1D and 2D-NMR, MS, UV, and IR data. It is worth mentioning that, some of the isolated linalol glycoside derivatives were reported as aroma precursors.
研究了花朵的总提取物与标准药物水飞蓟素在两个剂量下可能的肝保护活性。较高剂量表现出有前景的活性，特别是在降低天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和胆红素水平方面。通过液 - 液分配进行分馏并重新检测各馏分，结果显示正丁醇馏分在改善肝脏生化参数方面效果最佳，其次是正己烷馏分。然而，氯仿馏分对血清脂质参数的改善效果最佳。这些有前景的生物活性结果促使对花朵馏分进行深入的色谱纯化。首次从天然来源鉴定出两种化合物，无环法呢基倍半萜糖苷1 - O - [6 - O - α - l - 阿拉伯吡喃糖基 - β - d - 葡萄糖吡喃糖苷] - (2,6 - E) - 法呢醇（）和角鲨烯衍生物2,3 - 二羟基 - 2,3 - 二氢角鲨烯（），此外还有首次从该属报道的八种化合物；两种苄基糖苷，苄基1 - O - β - d - 葡萄糖吡喃糖苷（）和苄基6 - O - α - l - 阿拉伯吡喃糖基 - β - d - 葡萄糖吡喃糖苷（）；三种无环单萜糖苷，芳樟醇β - d - 葡萄糖吡喃糖苷（）和芳樟醇6 - O - α - l - 阿拉伯吡喃糖基 - β - d - 葡萄糖吡喃糖苷（）；(2) - 3,7 - 二甲基辛 - 2,6 - 二烯酸酯 - 6 - O - α - l - 阿拉伯吡喃糖基 - β - d - 葡萄糖吡喃糖苷（），两种寡糖苷，α - 己基 - α - l - 阿拉伯吡喃糖基 - (1→6) - β - d - 葡萄糖吡喃糖苷（creoside）（）和α - 辛基 - α - l - 阿拉伯吡喃糖基 - (1→6) - β - d - 葡萄糖吡喃糖苷（rhodiooctanoside）（）；以及乙基呋喃果糖苷（）。基于对其光谱1D和2D - NMR、MS、UV和IR数据的广泛检测，阐明了分离出的化合物的结构。值得一提的是，一些分离出的芳樟醇糖苷衍生物被报道为香气前体。
