Bioenginering Department, Faculty of Engineering, Cyprus International University, Via Mersin 10, Nicosia 98258, Northern Cyprus, Turkey.
Biotechnology Research Centre, Cyprus International University, Via Mersin 10, Nicosia 99258, Northern Cyprus, Turkey.
Molecules. 2021 Jun 24;26(13):3864. doi: 10.3390/molecules26133864.
Nano-based particles synthesized via green routes have a particular structure that is useful in biomedical applications as they provide cheap, eco-friendly, and non-toxic nanoparticles. In the present study, we reported the effect of various concentrations of Zinc oxide nanoparticles synthesized using stem bark extract (ZnO NPsAL) as stabilizing agent on rat biochemical profiles and tissue morphology. Adult Wistar rats weighing 170 ± 5 g were randomly classified into eight groups of five rats each; Group A served as a control fed with normal diet and water. Groups B1, B2, C1, C2, D1, D2, and E were treated with 40 mg/kg and 80 mg/kg of the 0.01, 0.05, and 0.1 M biosynthesized ZnO NPsAL and zinc nitrate daily by the gavage method, respectively. The rats were anesthetized 24 h after the last treatment, blood samples, kidney, heart, and liver tissues were collected for biochemical and histopathological analysis. The rats mean body weight, serum alkaline phosphatase, alanine aminotransferase, creatinine, urea, bilirubin, protein, albumin, globulin, total cholesterol, triacylglycerol, and high-density lipoprotein were significantly altered with an increased concentration of biosynthesized ZnO NPsAL when compared with the control group ( < 0.05; ≥ 5). Furthermore, histopathological analysis of treated rats' kidney, heart, and liver tissue revealed vascular congestion, tubular necrosis, inflammation, and cytoplasmic vacuolation. Biosynthesized ZnO NPsAL showed significant alteration in biochemical parameters and tissue morphology in rats with increasing concentrations of the nanoparticles.
基于纳米的粒子通过绿色途径合成，具有特殊的结构，在生物医学应用中非常有用，因为它们提供了廉价、环保和无毒的纳米粒子。在本研究中，我们报道了使用 茎皮提取物（ZnO NPsAL）作为稳定剂合成的不同浓度的氧化锌纳米粒子（ZnO NPsAL）对大鼠生化谱和组织形态的影响。170 ± 5 g 的成年 Wistar 大鼠随机分为 8 组，每组 5 只；A 组作为对照，给予正常饮食和水。B1、B2、C1、C2、D1、D2 和 E 组分别用 40 mg/kg 和 80 mg/kg 的 0.01、0.05 和 0.1 M 生物合成的 ZnO NPsAL 和硝酸锌灌胃。末次处理后 24 h 麻醉大鼠，采集血样、肾、心、肝组织进行生化和组织病理学分析。与对照组相比，大鼠的平均体重、血清碱性磷酸酶、丙氨酸氨基转移酶、肌酐、尿素、胆红素、蛋白、白蛋白、球蛋白、总胆固醇、三酰甘油和高密度脂蛋白均显著改变，生物合成的 ZnO NPsAL 浓度增加（<0.05；≥5）。此外，处理大鼠的肾、心、肝组织的组织病理学分析显示血管充血、肾小管坏死、炎症和细胞质空泡化。随着纳米粒子浓度的增加，生物合成的 ZnO NPsAL 对大鼠的生化参数和组织形态有显著改变。
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