van Ulsen J, Dumas A M, Wagenvoort J H, van Zuuren A, van Joost T, Stolz E
Department of Dermato-Venereology, University Hospital Rotterdam-Dijkzigt, The Netherlands.
Eur J Clin Microbiol. 1987 Aug;6(4):410-3. doi: 10.1007/BF02013096.
To evaluate a non-marketed research prototype of a solid-phase enzyme immunoassay for detection of herpes simplex virus in genital lesions, 154 clinical specimens were collected from 127 men and 27 women with symptoms suggestive of herpes simplex virus infection (erythema, vesicles, ulcers and crustae). The samples were tested using the assay and cultures on four monolayers of human embryonic lung fibroblasts and Vero cells. When the culture was used as reference method, sensitivity was 76.9% and specificity 100% (prevalence 42.4%). Comparison of results by patient group showed that sensitivity was highest in material from patients with vesicles and ulcers. The highest sensitivity was obtained in specimens which developed a cytopathological effect within 48 h and in specimens with three or four positive cell cultures. These findings suggest that the assay is more successful in specimens with high virus titres. The enzyme immunoassay was found to be a rapid, moderately sensitive, highly specific test for detection of herpes simplex virus from genital lesions, but the usefulness of the assay is limited and culture methods should be preferred.
为评估一种用于检测生殖器病变中单纯疱疹病毒的固相酶免疫测定法的非上市研究原型，从127名男性和27名女性中收集了154份临床标本，这些患者有提示单纯疱疹病毒感染的症状（红斑、水疱、溃疡和结痂）。使用该测定法以及在四层人胚肺成纤维细胞和非洲绿猴肾细胞单层上进行培养对样本进行检测。当以培养法作为参考方法时，敏感性为76.9%，特异性为100%（患病率为42.4%）。按患者组比较结果显示，水疱和溃疡患者的样本中敏感性最高。在48小时内出现细胞病理学效应的标本以及有三次或四次阳性细胞培养的标本中获得了最高敏感性。这些发现表明该测定法在病毒滴度高的标本中更成功。酶免疫测定法被发现是一种用于从生殖器病变中检测单纯疱疹病毒的快速、中度敏感、高度特异的检测方法，但该测定法的实用性有限，应优先选择培养方法。
