Kurtenbach A, Kramer S, Strasser T, Zrenner E, Langrová H
Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Elfriede-Aulhorn Strasse 7, 72076, Tübingen, Germany.
Werner Reichardt Centre for Integrative Neuroscience (CIN), University of Tübingen, Tübingen, Germany.
Doc Ophthalmol. 2017 Apr;134(2):129-134. doi: 10.1007/s10633-017-9579-9. Epub 2017 Feb 21.
The DTL fibre electrode is commonly used to record the electric potentials elicited by stimulation of the retina. Two positions are commonly used: it is placed either on the cornea along the lower lid or in the conjunctival fornix. The PERG and OPs have previously been examined and compared under both conditions. The aim of this study was to examine the ERG, flicker response and on-off responses with differing electrode positions.
Before recruitment, all subjects underwent an ophthalmological examination. We enrolled 13 normal control subjects into the study aged 13-64 years, all with a visual acuity of ≥1.0. We recorded scotopic and photopic ERGs, flicker and on-off responses, for both electrode positions. On the first day, one eye had the electrode placed on the cornea along the lower lid and the other eye had it positioned in the conjunctival sac. On a second day, the recordings were repeated with the alternative electrode placements.
ERG, on-off and flicker responses were all smaller by between 20 and 25% when the DTL electrode was positioned in the conjunctival sac, compared to when it was positioned on the cornea, as did the scatter in the data points. This indicates that there is no advantage clinically for one or the other placement.
Our results confirm other reports examining the effect of electrode position on electrophysiological potentials. When recording with the DTL electrode, it is important to ensure that it is placed at the same position in repeat recordings or in multicentre trials and that it is stable and does not move during recording.
DTL纤维电极常用于记录视网膜刺激引发的电位。通常使用两个位置：沿下眼睑置于角膜上或置于结膜穹窿。之前已经在这两种情况下对图形视网膜电图（PERG）和振荡电位（OPs）进行了检查和比较。本研究的目的是在不同电极位置下检查视网膜电图（ERG）、闪烁反应和开-关反应。
在招募受试者之前，所有受试者均接受了眼科检查。我们招募了13名年龄在13至64岁之间的正常对照受试者，所有受试者的视力均≥1.0。我们记录了两种电极位置下的暗视和明视视网膜电图、闪烁反应和开-关反应。第一天，一只眼睛的电极沿下眼睑置于角膜上，另一只眼睛的电极置于结膜囊中。第二天，更换电极位置后重复记录。
与电极置于角膜上相比，当DTL电极置于结膜囊中时，视网膜电图、开-关反应和闪烁反应均减小了20%至25%，数据点的离散度也是如此。这表明在临床上这两种放置方式没有优势。
我们的结果证实了其他关于电极位置对电生理电位影响的报告。使用DTL电极进行记录时，重要的是要确保在重复记录或多中心试验中电极置于相同位置，并且在记录过程中电极稳定且不移动。
