Lobo João Paulo, Brescansin Catiane Pompilio, Santos-Weiss Izabella C R, Welter Marciane, Souza Emanuel Maltempi de, Rego Fabiane Gomes de Moraes, Picheth Geraldo, Alberton Dayane
Pós-Graduação em Ciências Farmacêuticas, Departamento de Análise Clínica, Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
Departamento de Bioquímica e Biologia Molecular, UFPR, Curitiba, PR, Brasil.
Arch Endocrinol Metab. 2017 May-Jun;61(3):233-237. doi: 10.1590/2359-3997000000238. Epub 2017 Feb 13.
Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes.
Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm).
No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations.
It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.
晚期糖基化终末产物（AGEs）参与糖尿病（DM）的发病机制及并发症。妊娠期糖尿病（GDM）的特征为血糖升高和氧化应激，这些都是与血清AGE高浓度相关的因素。本研究的目的是评估血清荧光AGE（F-AGE）方法作为妊娠期糖尿病筛查工具的实用性。
将225例GDM患者和217例健康孕妇（健康对照）的血清样本在磷酸盐缓冲盐水中稀释50倍，通过荧光分析（激发波长350nm/发射波长440nm）估算AGEs。
在GDM女性或健康对照中，以任意单位（UA/mL×104）表示的AGE浓度无显著差异（P>0.05）。此外，在妊娠期间（妊娠12 - 32周）F-AGE浓度无显著变化。仅GDM组的F-AGE与血清肌酐浓度呈正相关（r = 0.421；P<0.001）。
无法根据血清F-AGE浓度区分妊娠期糖尿病女性与健康对照。
