Kazeem Mutiu Idowu, Mayaki Ayuva Mercy, Ogungbe Bimpe Folashade, Ojekale Anthony Babajide
Department of Biochemistry, Faculty of Science, Lagos State University, PMB 0001, Ojo, Lagos, Nigeria.
Iran J Pharm Res. 2016 Winter;15(Suppl):37-44.
The side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. Therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of leaf, as well as its possible mode of inhibiting these enzymes. Acetone, aqueous and ethanolic extracts of leaf was subjected to standard enzymes' inhibitory assay using porcine pancreatic α-amylase and rat intestinal α-glucosidase. Results obtained showed that out of all the extracts tested, ethanolic and aqueous extracts possessed the best inhibition of α-amylase (IC 7.80 mg/mL) and α-glucosidase (3.25 mg/mL) respectively. The kinetic analysis of the mode of inhibition of these enzymes by the leaf extracts of , revealed that these extracts inhibited both enzymes in a non-competitive manner. It is speculated that the α-amylase and α-glucosidase inhibitory properties of leaf extracts of may be due to the presence of some phytochemicals such as flavonoids, tannins and saponins in the plant. It can be concluded from this study that the extracts could serve as source of antidiabetic agents which may act through the inhibition of carbohydrate hydrolyzing enzymes, α-amylase and α-glucosidase.
合成抗糖尿病药物的副作用使得从药用植物中寻找替代药物成为当务之急。因此，本研究旨在评估[植物名称]叶的α-淀粉酶和α-葡萄糖苷酶抑制潜力，以及其抑制这些酶的可能方式。使用猪胰腺α-淀粉酶和大鼠肠道α-葡萄糖苷酶对[植物名称]叶的丙酮、水和乙醇提取物进行标准酶抑制试验。所得结果表明，在所有测试提取物中，乙醇提取物和水提取物对α-淀粉酶（IC50为7.80mg/mL）和α-葡萄糖苷酶（IC50为3.25mg/mL）的抑制效果最佳。对[植物名称]叶提取物抑制这些酶的方式进行动力学分析，结果显示这些提取物以非竞争性方式抑制这两种酶。据推测，[植物名称]叶提取物的α-淀粉酶和α-葡萄糖苷酶抑制特性可能归因于该植物中存在一些植物化学物质，如黄酮类、单宁和皂苷。从本研究可以得出结论，[植物名称]提取物可作为抗糖尿病药物的来源，其可能通过抑制碳水化合物水解酶α-淀粉酶和α-葡萄糖苷酶发挥作用。
