Department of Biochemistry, Covenant University, Canaanland, P.M.B, 1023 Ota, Ogun State, Nigeria.
Covenant University Public Health and Wellbeing Research Cluster (CUPHWERC), Covenant University, Canaanland, P.M.B, 1023 Ota, Ogun State, Nigeria.
Oxid Med Cell Longev. 2020 Mar 9;2020:5612486. doi: 10.1155/2020/5612486. eCollection 2020.
This study was carried out to assess the antioxidant, anti-inflammatory and antidiabetic effects of (Sm.) leaf extracts. Ethanolic (NLE) and aqueous (NLA) extract of leaves were prepared and assessed for their anti-inflammatory activity, antioxidant potential, -amylase and -glucosidase inhibitory activities, and the mechanism of enzyme inhibition using standard established methods. From the results, phytochemicals such as flavonoids, phenolics, glycosides, and tannins were detected in both extracts of with NLE having a significantly ( < 0.05) higher phytochemical content. NLE displayed significantly ( < 0.05) better total antioxidant capacity, reducing power, 2,2-diphenyl-2-picrylhydrazyl, and hydrogen peroxide radical scavenging activities. For anti-inflammatory activities, 70.54 ± 2.45% albumin denaturation inhibition was observed for NLE while 68.05 ± 1.03% was recorded for NLA. Likewise, 16.07 ± 1.60 and 14.08 ± 1.76% were obtained against hypotonic solution and heat-induced erythrocyte haemolysis, respectively, for NLE while 20.59 ± 4.60 and 24.07 ± 1.60% were respective NLA values. NLE (IC: 4.20 ± 0.18 and 1.19 ± 0.11 mg/mL) and NLA (IC: 11.21 ± 0.35 and 2.64 ± 0.48 mg/mL) -glucosidase and -amylase inhibitory activities were dose-dependent with uncompetitive and competitive inhibition elicited, respectively, by the extracts. A significant positive association ( < 0.01 and 0.05) was identified between antioxidant activity and carbohydrate-metabolising enzyme inhibitory activity. The obtained result suggests leaf could serve as an alternative candidate for managing diabetes mellitus due to its antioxidant and anti-inflammatory association with diabetes-linked enzymes.
这项研究旨在评估（Sm.）叶提取物的抗氧化、抗炎和抗糖尿病作用。制备了乙醇（NLE）和水（NLA）提取物，并使用标准方法评估其抗炎活性、抗氧化潜力、-淀粉酶和 -葡萄糖苷酶抑制活性以及酶抑制机制。结果表明，两种提取物中均检测到类黄酮、酚类、糖苷和单宁等植物化学物质，其中 NLE 的植物化学物质含量明显更高（<0.05）。NLE 表现出显著（<0.05）更好的总抗氧化能力、还原能力、2,2-二苯基-2-苦基肼（DPPH）自由基清除能力和过氧化氢自由基清除能力。对于抗炎活性，NLE 观察到 70.54±2.45%的白蛋白变性抑制，而 NLA 则记录到 68.05±1.03%。同样，NLE 对低渗溶液和热诱导的红细胞溶血的抑制率分别为 16.07±1.60%和 14.08±1.76%，而 NLA 的相应值分别为 20.59±4.60%和 24.07±1.60%。NLE（IC：4.20±0.18 和 1.19±0.11mg/mL）和 NLA（IC：11.21±0.35 和 2.64±0.48mg/mL）对 -葡萄糖苷酶和 -淀粉酶的抑制活性呈剂量依赖性，提取物分别引起非竞争性和竞争性抑制。抗氧化活性与碳水化合物代谢酶抑制活性之间存在显著正相关（<0.01 和 0.05）。研究结果表明，由于其与糖尿病相关酶的抗氧化和抗炎关联，可能成为治疗糖尿病的替代候选药物。
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