Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
Institute of Biomedical Informatics and Center for Systems and Synthetic Biology, National Yang-Ming University, Taipei, Taiwan.
Appl Environ Microbiol. 2019 May 30;85(12). doi: 10.1128/AEM.02206-18. Print 2019 Jun 15.
, a coprophilous ascomycete, is a homothallic fungus that can undergo sexual differentiation with cellular and morphological changes followed by multicellular tissue development to complete its sexual cycle. In this study, we identified and characterized the blue-light photoreceptor gene in The white collar-1 photoreceptor (SfWC-1) contains light-oxygen-voltage-sensing (LOV), Per-Arnt-Sim (PAS), and other conserved domains and is homologous to the WC-1 blue-light photoreceptor of The LOV domain of was deleted by homologous recombination using -mediated protoplast transformation. The mutant showed normal vegetative growth but produced less carotenoid pigment under illumination. The mutant showed delayed and less-pronounced fruiting-body formation, was defective in phototropism of the perithecial beaks, and lacked the fruiting-body zonation pattern compared with the wild type under the illumination condition. Gene expression analyses supported the light-induced functions of the gene in the physiology and developmental process of perithecial formation in Moreover, green fluorescent protein (GFP)-tagged SfWC-1 fluorescence signals were transiently strong upon light induction and prominently located inside the nuclei of living hyphae. Our studies focused on the putative blue-light photoreceptor in a model ascomycete and contribute to a better understanding of the photoregulatory functions and networks mediated by the evolutionarily conserved blue-light photoreceptors across diverse fungal phyla. sp. has been a model for study of fruiting-body differentiation in fungi. Several environmental factors, including light, affect cellular and morphological changes during multicellular tissue development. Here, we created a light-oxygen-voltage-sensing (LOV) domain-deleted mutant to study blue-light photoresponses in Phototropism and rhythmic zonation of perithecia were defective in the mutant. Moreover, fruiting-body development in the mutant was reduced and also significantly delayed. Gene expression analysis and subcellular localization study further revealed the light-induced differential gene expression and cellular responses upon light stimulation in .
，一种腐生性子囊菌，是一种同宗配合真菌，能够进行性分化，伴随着细胞和形态变化，然后进行多细胞组织发育，完成其有性周期。在这项研究中，我们鉴定并描述了蓝光照感基因在中的特征。白色领结-1 光受体（SfWC-1）含有光-氧-电压感应（LOV）、Per-Arnt-Sim（PAS）和其他保守结构域，与拟南芥 WC-1 蓝光照感蛋白同源。通过 -介导的原生质体转化，利用同源重组删除了 SfWC-1 的 LOV 结构域。该突变体表现出正常的营养生长，但在光照下产生的类胡萝卜素色素较少。与野生型相比，突变体在光照条件下形成的子实体延迟且不明显，喙状子实体的光向性缺陷，且缺乏子实体分带模式。基因表达分析支持 SfWC-1 基因在光照诱导的子实体形成过程中的生理和发育过程中的功能。此外，绿色荧光蛋白（GFP）标记的 SfWC-1 荧光信号在光诱导下短暂强烈，并且主要位于活菌丝的细胞核内。我们的研究集中在模式子囊菌中的假定蓝光照感蛋白上，有助于更好地理解不同真菌门中进化保守的蓝光照感蛋白介导的光调控功能和网络。 sp. 一直是研究真菌体分化的模式生物。几种环境因素，包括光，会影响多细胞组织发育过程中的细胞和形态变化。在这里，我们创建了一个 LOV 结构域缺失的突变体，以研究 在 SfWC-1 突变体中，光向性和周期性的子实体分带发生缺陷。此外，突变体的子实体发育减少，且显著延迟。基因表达分析和亚细胞定位研究进一步揭示了光照刺激下基因表达的差异和细胞反应。
