Matsui K, Sano K, Ohtsubo E
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan.
Mol Gen Genet. 1987 Sep;209(2):299-305. doi: 10.1007/BF00329657.
Brevibacterium lactofermentum, a Gram-positive bacterium, is a commercially important amino acid producer. In this organism, the tryptophan biosynthetic enzymes are encoded within a 7725 bp HapII-BamHI fragment. Seven open reading frames were identified as trp genes by complementation tests with various B. lactofermentum and Escherichia coli tryptophan auxotrophs. Following the nomenclature established for E. coli and Serratia marcescens, the B. lactofermentum trp genes were designated trpL, trpE, trpG, trpD, trpC (including the trpF domain), trpB, and trpA. The organization of these genes is identical to that in S. marcescens. The nucleotide sequences of the putative ribosome-binding sites for the B. lactofermentum trp genes resemble those of E. coli and Bacillus subtilis. Computer analysis revealed that the trp enzymes of B. lactofermentum resemble the enzymes of the Gram-negative E. coli more closely than those of the Gram-positive B. subtilis.
乳酸发酵短杆菌是一种革兰氏阳性菌，是一种具有重要商业价值的氨基酸生产菌。在这种生物体中，色氨酸生物合成酶由一个7725 bp的HapII - BamHI片段编码。通过与各种乳酸发酵短杆菌和大肠杆菌色氨酸营养缺陷型进行互补试验，鉴定出7个开放阅读框为色氨酸基因。按照为大肠杆菌和粘质沙雷氏菌建立的命名法，乳酸发酵短杆菌的色氨酸基因被命名为trpL、trpE、trpG、trpD、trpC（包括trpF结构域）、trpB和trpA。这些基因的组织方式与粘质沙雷氏菌中的相同。乳酸发酵短杆菌色氨酸基因假定的核糖体结合位点的核苷酸序列与大肠杆菌和枯草芽孢杆菌的相似。计算机分析表明，乳酸发酵短杆菌的色氨酸酶与革兰氏阴性的大肠杆菌的酶比与革兰氏阳性的枯草芽孢杆菌的酶更相似。
