Hahn B H, Kong L I, Lee S W, Kumar P, Taylor M E, Arya S K, Shaw G M
Department of Internal Medicine, University of Alabama, Birmingham 35294.
Nature. 1987;330(6144):184-6. doi: 10.1038/330184a0.
Human immunodeficiency virus type 1 (HIV-1) is the aetiologic agent of AIDS (acquired immune deficiency syndrome) in most countries and probably originated in Central Africa like the AIDS epidemic itself. Evidence for a second major group of human immunodeficiency-associated retroviruses came from a report that West African human populations like wild-caught African green monkeys had serum antibodies that reacted more strongly with a simian immunodeficiency virus (STLV-3Mac) (ref.6) than with HIV-1. Novel T-lymphotropic retroviruses were reported to have been isolated from healthy Senegalese West Africans (HTLV-4) (ref. 4) and from African green monkeys (STLV-3AGM) (ref. 7), and a different retrovirus (HIV-2) was identified in other West African AIDS patients. Genomic analysis of HIV-2 clearly distinguished it from STLV-3 (ref. 9), but restriction enzyme site-mapping of three different HTLV-4 isolates and six different STLV-3AGM isolates showed them to be essentially indistinguishable. In this report we clone, restriction map, and partially sequence three isolates of HTLV-4 (PK82, PK289, PK190) (ref. 4). We find that these viruses differ in nucleotide sequence from each other and from three isolates of STLV-3AGM (K78, K6W, K1) (ref. 7) by 1% or less. We also report the isolation of a T-lymphotropic retrovirus from the peripheral blood of a healthy Senegalese woman which hybridizes preferentially to HIV-2 specific DNA probes. We conclude that HTLV-4 (ref. 4) and STLV-3AGM (ref. 7) are not independent virus isolates and that HIV-2 is present in Senegal as it is in other West African countries.
在大多数国家，人类免疫缺陷病毒1型（HIV-1）是获得性免疫缺陷综合征（AIDS，艾滋病）的病原体，它可能像艾滋病疫情本身一样起源于中非。人类免疫缺陷相关逆转录病毒第二大主要组别的证据来自一份报告，该报告指出，西非人群，如野生捕获的非洲绿猴，其血清抗体与猿猴免疫缺陷病毒（STLV-3Mac）（参考文献6）的反应比与HIV-1的反应更强。据报道，新型嗜T淋巴细胞逆转录病毒已从健康的塞内加尔西非人中分离出来（HTLV-4）（参考文献4），也从非洲绿猴中分离出来（STLV-3AGM）（参考文献7），并且在其他西非艾滋病患者中鉴定出一种不同的逆转录病毒（HIV-2）。对HIV-2的基因组分析清楚地将其与STLV-3区分开来（参考文献9），但对三种不同的HTLV-4分离株和六种不同的STLV-3AGM分离株进行的限制酶位点图谱分析表明，它们基本上无法区分。在本报告中，我们对HTLV-4的三个分离株（PK82、PK289、PK190）（参考文献4）进行了克隆、限制酶图谱绘制和部分测序。我们发现这些病毒彼此之间以及与三种STLV-3AGM分离株（K78、K6W、K1）（参考文献7）的核苷酸序列差异为1%或更小。我们还报告了从一名健康的塞内加尔妇女外周血中分离出一种嗜T淋巴细胞逆转录病毒，它优先与HIV-2特异性DNA探针杂交。我们得出结论，HTLV-4（参考文献4）和STLV-3AGM（参考文献7）不是独立的病毒分离株，并且HIV-2在塞内加尔与在其他西非国家一样存在。
