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鉴定一种人类免疫缺陷病毒2型和猿猴免疫缺陷病毒SIVMAC特有的新型逆转录病毒基因。

Identification of a novel retroviral gene unique to human immunodeficiency virus type 2 and simian immunodeficiency virus SIVMAC.

作者信息

Kappes J C, Morrow C D, Lee S W, Jameson B A, Kent S B, Hood L E, Shaw G M, Hahn B H

机构信息

Department of Medicine, University of Alabama, Birmingham 35294.

出版信息

J Virol. 1988 Sep;62(9):3501-5. doi: 10.1128/JVI.62.9.3501-3505.1988.

DOI:10.1128/JVI.62.9.3501-3505.1988
PMID:3136256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253477/
Abstract

Human and simian immunodeficiency-associated retroviruses are extraordinarily complex, containing at least five genes, tat, art, sor, R, and 3' orf, in addition to the structural genes gag, pol, and env. Recently, nucleotide sequence analysis of human immunodeficiency virus type 2 (HIV-2) and simian immunodeficiency virus SIVMAC revealed the existence of still another open reading frame, termed X, which is highly conserved between these two viruses but absent from HIV-1. In this report, we demonstrate for the first time that the X open reading frame represents a functional retroviral gene in both HIV-2 and SIVMAC and that it encodes a virion-associated protein of 14 and 12 kilodaltons, respectively. We also describe the production of recombinant TrpE/X fusion proteins in Escherichia coli and show that sera from some HIV-2-infected individuals specifically recognize these proteins.

摘要

人类和猿猴免疫缺陷相关逆转录病毒极其复杂，除结构基因gag、pol和env外，还至少包含五个基因，即tat、art、sor、R和3'orf。最近，对2型人类免疫缺陷病毒（HIV-2）和猿猴免疫缺陷病毒SIVMAC的核苷酸序列分析显示，还存在另一个开放阅读框，称为X，它在这两种病毒之间高度保守，但在HIV-1中不存在。在本报告中，我们首次证明X开放阅读框在HIV-2和SIVMAC中均代表一个功能性逆转录病毒基因，并且它分别编码14千道尔顿和12千道尔顿的病毒体相关蛋白。我们还描述了重组TrpE/X融合蛋白在大肠杆菌中的产生，并表明来自一些HIV-2感染个体的血清能特异性识别这些蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/2630ff9305a1/jvirol00088-0441-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/d4ab382da70c/jvirol00088-0440-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/2943d3aed945/jvirol00088-0441-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/2630ff9305a1/jvirol00088-0441-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/d4ab382da70c/jvirol00088-0440-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/2943d3aed945/jvirol00088-0441-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d28b/253477/2630ff9305a1/jvirol00088-0441-b.jpg

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