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杜洛克、约克夏、长白和汉普夏公猪的粪便微生物群组成。

The fecal microbiota composition of boar Duroc, Yorkshire, Landrace and Hampshire pigs.

作者信息

Xiao Yingping, Li Kaifeng, Xiang Yun, Zhou Weidong, Gui Guohong, Yang Hua

机构信息

Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

出版信息

Asian-Australas J Anim Sci. 2017 Oct;30(10):1456-1463. doi: 10.5713/ajas.16.0746. Epub 2017 Feb 23.

DOI:10.5713/ajas.16.0746
PMID:28231697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5582331/
Abstract

OBJECTIVE

To investigate the effect of host genetics on gut microbial diversity, we performed a structural survey of the fecal microbiota of four purebred boar pig lines: Duroc, Landrace, Hampshire, and Yorkshire.

METHODS

The V3-V4 regions of the 16S rRNA genes were amplified and sequenced.

RESULTS

A total of 783 operational taxonomic units were shared by all breeds, whereas others were breed-specific. Firmicutes and Bacteroidetes dominated the majority of the fecal microbiota; Clostridia, Bacilli, and Bacteroidia were the major classes. Nine predominant genera were observed in all breeds and eight of them can produce short-chain fatty acids. Some bacteria can secrete cellulase to aid fiber digestion by the host. Butyric, isobutyric, valeric, and isovaleric acid levels were highest in Landrace pigs, whereas acetic and propionic acid were highest in the Hampshire breed. Heatmap was used to revealed breed-specific bacteria. Principal coordinate analysis of fecal bacteria revealed that the Landrace and Yorkshire breeds had high similarity and were clearly separated from the Duroc and Hampshire breeds.

CONCLUSION

Overall, this study is the first time to compare the fecal microbiomes of four breeds of boar pig by high-throughput sequencing and to use Spearman's rank correlation to analyze competition and cooperation among the core bacteria.

摘要

目的

为了研究宿主基因对肠道微生物多样性的影响，我们对四个纯种公猪品系（杜洛克、长白、汉普夏和约克夏）的粪便微生物群进行了结构调查。

方法

对16S rRNA基因的V3-V4区域进行扩增和测序。

结果

所有品种共有的可操作分类单元有783个，其他的则具有品种特异性。厚壁菌门和拟杆菌门在大多数粪便微生物群中占主导地位；梭菌纲、芽孢杆菌纲和拟杆菌纲是主要类别。在所有品种中观察到9个优势属，其中8个可以产生短链脂肪酸。一些细菌可以分泌纤维素酶以帮助宿主消化纤维物质。长白猪的丁酸、异丁酸、戊酸和异戊酸水平最高，而汉普夏品种的乙酸和丙酸水平最高。使用热图来揭示品种特异性细菌。粪便细菌的主坐标分析表明，长白和约克夏品种具有高度相似性，并且与杜洛克和汉普夏品种明显分开。

结论

总体而言，本研究首次通过高通量测序比较了四种公猪品种的粪便微生物群，并使用Spearman等级相关性分析了核心细菌之间的竞争与合作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/58d96e64b60d/ajas-30-10-1456f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/155639bc7c25/ajas-30-10-1456f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/67cd0916d36d/ajas-30-10-1456f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/3106a0d7b215/ajas-30-10-1456f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/e0934ba662eb/ajas-30-10-1456f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/e44b47d81dab/ajas-30-10-1456f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/0b1280b548be/ajas-30-10-1456f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/58d96e64b60d/ajas-30-10-1456f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/155639bc7c25/ajas-30-10-1456f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/67cd0916d36d/ajas-30-10-1456f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/3106a0d7b215/ajas-30-10-1456f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/e0934ba662eb/ajas-30-10-1456f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/e44b47d81dab/ajas-30-10-1456f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/0b1280b548be/ajas-30-10-1456f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1050/5582331/58d96e64b60d/ajas-30-10-1456f7.jpg

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2
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3
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4
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5
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6
Characterization of microbiota signatures in Iberian pig strains using machine learning algorithms.使用机器学习算法对伊比利亚猪品系中的微生物群特征进行表征。
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7
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6
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7
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8
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9
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