Feng Luya, Luo Zhenfu, Wang Jing, Wu Kunfu, Wang Wenliang, Liu Zhimou, Wen Juping, Wang Zhenbin, Duns Gregory J, Ma Xiaokang, Tan Bi'e
Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Yuelushan Laboratory, Changsha 410128, China.
Anim Nutr. 2024 Jul 10;18:257-271. doi: 10.1016/j.aninu.2024.05.005. eCollection 2024 Sep.
This study investigated the impact of different ratios of soluble to insoluble dietary fiber (SDF:IDF) formulations by sugar beet pulp (SBP) supplementation on piglet growth performance, nutrient digestibility, immune function, intestinal morphology, intestinal microbiota and intestinal health. A total of 60 crossbred piglets (Duroc × [Landrace × Yorkshire]) at 40 d old with body weight of 10.0 ± 0.3 kg were randomly assigned to 5 treatments with 6 replicates per treatment and 2 piglets per replicate in a 21-d trial. The dietary treatments included a corn-soybean meal diet (0% SBP supplementation; CON), and diets supplemented with 2%, 4%, 6%, and 8% SBP, representing different SDF:IDF ratios at 10.16%, 13.53%, 16.79%, 19.86%, and 24.81%, respectively. The results indicated that the 8% SBP treatment had a negative effect on feed-to-gain ratio (linear, = 0.009) compared with the CON treatment ( = 0.021). The apparent total tract digestibility (ATTD) of crude protein was lower in treatments supplemented with SBP ( = 0.002) and showed a linear decrease ( = 0.001), while the ATTD of IDF showed a linear increase ( = 0.037) in four SBP treatments compared to the CON treatment. The 4% SBP treatment increased serum concentrations of triglyceride (quadratic, = 0.019) and K (linear, < 0.0037), and decreased alanine transaminase concentration (quadratic, = 0.015) compared with the CON treatment. The concentrations of Cit, Cys, Ile, Leu, Orn, Arg, taurine, urea, 1-methylhistidine, α-aminoadipic acid, α-aminobutyric acid and cystathionine in the 4% SBP treatment were highest among all treatments ( < 0.05). The serum concentrations of interleukin-6, interleukin-8, interleukin-10, transforming growth factor-β, and tumor necrosis factor-α in the 6% SBP treatment were higher than those in the CON treatment ( < 0.05), which also increased mucin-2 and G protein-coupled receptor 41 mRNA expression ( < 0.05) in colonic mucosa compared with the CON treatment and improved the intestinal barrier function. Diets containing more than 19.86% SDF:IDF could impair the intestinal health in piglets when SBP was used as the SDF source. Supplementing nursery piglet diets with 16.79% to 19.86% SDF:IDF is recommended for improving intestinal barrier function, increasing short-chain fatty acids concentrations, and improving intestinal microbiota composition.
本研究调查了添加甜菜粕（SBP）对仔猪生长性能、养分消化率、免疫功能、肠道形态、肠道微生物群和肠道健康的影响，其中不同比例的可溶性膳食纤维与不可溶性膳食纤维（SDF:IDF）配方由SBP补充剂提供。在一项为期21天的试验中，将60头40日龄、体重10.0±0.3千克的杜洛克×（长白×约克夏）杂交仔猪随机分为5组处理，每组6个重复，每个重复2头仔猪。日粮处理包括玉米-豆粕型基础日粮（不添加SBP；CON），以及分别添加2%、4%、6%和8% SBP的日粮，其SDF:IDF比例分别为10.16%、13.53%、16.79%、19.86%和24.81%。结果表明，与CON处理相比，8% SBP处理对料重比有负面影响（线性，P = 0.009；二次项，P = 0.021）。补充SBP的处理中粗蛋白的表观全肠道消化率（ATTD）较低（P = 0.002）且呈线性下降（P = 0.001），而与CON处理相比，在四种SBP处理中IDF的ATTD呈线性增加（P = 0.037）。与CON处理相比，4% SBP处理提高了血清甘油三酯浓度（二次项，P = 0.019）和钾浓度（线性，P < 0.0037），并降低了谷丙转氨酶浓度（二次项，P = 0.015）。4% SBP处理中Cit、Cys、Ile、Leu、Orn、Arg、牛磺酸、尿素、1-甲基组氨酸、α-氨基己二酸、α-氨基丁酸和胱硫醚的浓度在所有处理中最高（P < 0.05）。6% SBP处理的血清白细胞介素-6、白细胞介素-8、白细胞介素-10、转化生长因子-β和肿瘤坏死因子-α浓度高于CON处理（P < 0.05），与CON处理相比，其结肠黏膜中黏蛋白-2和G蛋白偶联受体41 mRNA表达也增加（P < 0.05），并改善了肠道屏障功能。当以SBP作为SDF来源时，SDF:IDF超过19.86%的日粮可能会损害仔猪的肠道健康。建议在保育仔猪日粮中添加16.79%至19.86%的SDF:IDF，以改善肠道屏障功能、提高短链脂肪酸浓度并改善肠道微生物群组成。
