Sanchez C, Bay-Jensen A-C, Pap T, Dvir-Ginzberg M, Quasnichka H, Barrett-Jolley R, Mobasheri A, Henrotin Y
Bone and Cartilage Research Unit, Arthropôle Liège, University of Liège, CHU Sart-Tilman, Belgium; The D-BOARD European Consortium for Biomarker Discovery.
The D-BOARD European Consortium for Biomarker Discovery; Department of Rheumatology, Biomarkers and Research, Nordic Bioscience, Herlev Hovedgade 207, 2730, Herlev, Denmark.
Osteoarthritis Cartilage. 2017 Aug;25(8):1199-1209. doi: 10.1016/j.joca.2017.02.797. Epub 2017 Feb 20.
The extracellular matrix (ECM) of articular cartilage is comprised of complex networks of proteins and glycoproteins, all of which are expressed by its resident cell, the chondrocyte. Cartilage is a unique tissue given its complexity and ability to resist repeated load and deformation. The mechanisms by which articular cartilage maintains its integrity throughout our lifetime is not fully understood, however there are numerous regulatory pathways known to govern ECM turnover in response to mechanical stimuli. To further our understanding of this field, we envision that proteomic analysis of the secretome will provide information on how the chondrocyte remodels the surrounding ECM in response to load, in addition to providing information on the metabolic state of the cell. In this review, we attempt to summarize the recent mass spectrometry-based proteomic discoveries in healthy and diseased cartilage and chondrocytes, to facilitate the discovery of novel biomarkers linked to degenerative pathologies, such as osteoarthritis (OA).
关节软骨的细胞外基质（ECM）由蛋白质和糖蛋白的复杂网络组成，所有这些都是由其驻留细胞软骨细胞表达的。软骨是一种独特的组织，因其复杂性以及抵抗反复负荷和变形的能力。关节软骨在我们一生中维持其完整性的机制尚未完全了解，然而，已知有许多调节途径来控制ECM周转以响应机械刺激。为了进一步了解该领域，我们设想对分泌组进行蛋白质组学分析，除了提供有关细胞代谢状态的信息外，还将提供有关软骨细胞如何响应负荷重塑周围ECM的信息。在这篇综述中，我们试图总结最近基于质谱的蛋白质组学在健康和患病软骨及软骨细胞中的发现，以促进与退行性疾病（如骨关节炎（OA））相关的新型生物标志物的发现。
