Green K Y, Midthun K, Gorziglia M, Hoshino Y, Kapikian A Z, Chanock R M, Flores J
Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Virology. 1987 Nov;161(1):153-9. doi: 10.1016/0042-6822(87)90181-4.
We sequenced the gene coding for the major neutralizing protein (VP7) from eight human rotavirus strains representing serotype 1, 2, 3, or 4. In addition, the corresponding gene of the rhesus rotavirus vaccine strain MMU 18006 (serotype 3) was sequenced. Comparative analyses of their deduced amino acid sequences revealed an overall 15-29% divergence in the VP7 proteins that define four different rotavirus serotypes and confirmed the presence of six discrete regions of clustered sequence divergence (amino acids 39-50, 87-101, 120-130, 143-152, 208-221, and 233-242). When the same regions were compared among rotaviruses belonging to the same serotype, a high degree of homology (91-99%) was detected. These observations indicate that differences in the serotype specificity among rotaviruses are the result of a high degree of sequence divergence in several discrete regions of the VP7 gene and that these regions are highly conserved within a given serotype.
我们对代表1、2、3或4型的8株人轮状病毒株编码主要中和蛋白（VP7）的基因进行了测序。此外，还对恒河猴轮状病毒疫苗株MMU 18006（3型）的相应基因进行了测序。对其推导的氨基酸序列进行比较分析发现，定义四种不同轮状病毒血清型的VP7蛋白总体上有15% - 29%的差异，并证实存在六个离散的序列差异聚集区域（氨基酸39 - 50、87 - 101、120 - 130、143 - 152、208 - 221和233 - 242）。当对属于同一血清型的轮状病毒之间的相同区域进行比较时，检测到高度的同源性（91% - 99%）。这些观察结果表明，轮状病毒血清型特异性的差异是VP7基因几个离散区域高度序列差异的结果，并且这些区域在给定的血清型内高度保守。
