Huang J, Nagesha H S, Dyall-Smith M L, Holmes I H
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Arch Virol. 1989;109(3-4):173-83. doi: 10.1007/BF01311079.
The genes coding for the rotavirus major neutralizing protein, VP7, from 5 Australian porcine rotaviruses representing glycoprotein (i.e. VP7 or G) serotypes 3, 4, and 5, were sequenced. The genes were each 1,062 nucleotides long with two long open reading frames for proteins of either 326 or 297 amino acids and containing only one potential glycosylation site at amino acid position 69. When compared to the corresponding genes of human viruses, the porcine genes showed very high nucleotide and deduced amino acid homology. Sequence comparison also revealed that Australian porcine rotaviruses of G serotype 4 and 5 were similar to the corresponding porcine strains found in the U.S.A. and U.K., while G serotype 3 and 4 porcine rotaviruses were closely related to human G serotype 3 strain, RV-3 and serotype 4 strain, ST-3, respectively. These Australian rotavirus VP7 sequences were found to correlate with serological data we reported previously.
对来自5株澳大利亚猪轮状病毒的轮状病毒主要中和蛋白VP7的编码基因进行了测序，这些病毒代表糖蛋白（即VP7或G）血清型3、4和5。这些基因各长1062个核苷酸，有两个长开放阅读框，分别编码326或297个氨基酸的蛋白质，且仅在氨基酸位置69处有一个潜在的糖基化位点。与人类病毒的相应基因相比，猪基因显示出非常高的核苷酸和推导氨基酸同源性。序列比较还显示，澳大利亚G血清型4和5的猪轮状病毒与在美国和英国发现的相应猪毒株相似，而G血清型3和4的猪轮状病毒分别与人类G血清型3毒株RV-3和血清型4毒株ST-3密切相关。发现这些澳大利亚轮状病毒VP7序列与我们先前报道的血清学数据相关。
