Visseaux Benoit, Collin Gilles, Ichou Houria, Charpentier Charlotte, Bendhafer Samia, Dumitrescu Madalina, Allal Lahcene, Cojocaru Bogdan, Desfrère Luc, Descamps Diane, Mandelbrot Laurent, Houhou-Fidouh Nadhira
IAME, UMR 1137, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Virologie, Hôpital Bichat, AP-HP, Paris, France.
Service de Néonatologie, Hôpital Louis Mourier, AP-HP, Colombes, France.
PLoS One. 2017 Feb 24;12(2):e0172809. doi: 10.1371/journal.pone.0172809. eCollection 2017.
To date, only influenza and RSV testing are recommended for respiratory viruses' detection in paediatric units. In this study, we described, according to seasons, ages and clinical units, the results obtained in children (<15 years old) by multiplex-PCR (mPCR) tests allowing a quick and wide range detection of all respiratory viruses. These results were also compared with RSV specific detection.
All nasopharyngeal mPCR and RSV tests requested by clinicians in our French teaching hospitals group between 2011 and 2014 were retrospectively included. All repeated samples for the same children in the same month were discarded.
Of the 381 mPCR tests (344 children) performed, 51.4% were positive. Positivity and viral co-infection rates were higher in the 6-36 months old strata (81% and 25%, p<0.0001 and p = 0.04, respectively). Viral distribution showed strong variations across ages. During specific influenza epidemic periods, only 1/39 (2.5%) mPCR tests were positive for influenza and 19/39 (48.7%) for other viruses. During specific RSV epidemic periods, only 8/46 (17.4%) mPCR tests were positive for RSV and 14/46 (30.4%) for other viruses. 477/1529 (31.2%) of RSV immunochromatography-tests were positive. Among the negatives immunochromatography-test also explored by mPCR, 28/62 (31%) were positive for other respiratory viruses.
This study provides a wide description of respiratory viruses' distribution among children in hospital settings using mPCR over 3 years. It emphasizes the number of undiagnosed respiratory viruses according to the current diagnosis practice in France and gives a better picture of respiratory viruses identified in hospital settings by mPCR all over the year in France.
迄今为止，儿科病房中呼吸道病毒检测仅推荐进行流感和呼吸道合胞病毒（RSV）检测。在本研究中，我们根据季节、年龄和临床科室，描述了通过多重聚合酶链反应（mPCR）检测在15岁以下儿童中获得的结果，该检测能够快速、广泛地检测所有呼吸道病毒。这些结果还与RSV特异性检测结果进行了比较。
回顾性纳入了2011年至2014年期间我们法国教学医院集团临床医生所要求的所有鼻咽部mPCR和RSV检测。同一个月内同一儿童的所有重复样本均被剔除。
在进行的381次mPCR检测（涉及344名儿童）中，51.4%呈阳性。6至36个月年龄组的阳性率和病毒合并感染率更高（分别为81%和25%，p<0.0001和p = 0.04）。病毒分布在各年龄段有很大差异。在特定的流感流行期间，mPCR检测中仅1/39（2.5%）对流感呈阳性，19/39（48.7%）对其他病毒呈阳性。在特定的RSV流行期间，mPCR检测中仅8/46（17.4%）对RSV呈阳性，14/46（30.4%）对其他病毒呈阳性。1529次RSV免疫层析检测中有477/1529（31.2%）呈阳性。在免疫层析检测为阴性且也通过mPCR检测的样本中，28/62（31%）对其他呼吸道病毒呈阳性。
本研究对3年间使用mPCR检测医院环境中儿童呼吸道病毒的分布情况进行了广泛描述。它强调了根据法国当前诊断实践未被诊断出的呼吸道病毒数量，并更清楚地呈现了法国全年通过mPCR在医院环境中鉴定出的呼吸道病毒情况。
